基于途径分析促进α-酮戊二酸过量积累的数学模型技术  

Enhancement of α-ketoglutaric Acid Production by Torulopsis glabrata Using Pathway Analysis and Math Model Technology

作　　者：孙丽平[1] 刘立明[1] 堵国成[1] 陈坚[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室

出　　处：《应用与环境生物学报》2008年第1期113-117,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金(No30670066);国家杰出青年基金(No20625619);长江学者和创新团队发展计划资助(NoIRT0532);国家高技术研究发展计划(“863”计划,2006AA02Z01)项目资助~~

摘　　要：在详尽分析α-酮戊二酸(α-KG)合成途径的基础上,结合数学模型,对光滑球拟酵母(Torulopsis glabrata)CCTCC M202019过量合成α-KG的合成途径及其调控因素进行全面分析与优化.全因子实验表明,硫胺素(B1)和Ca-CO3是影响α-KG过量积累的关键因素.在此基础上,采用最速上升实验得到α-酮戊二酸积累的最大响应区域为B10.016mg/L、CaCO384g/L附近.采用中心组合设计及响应面分析确定最优培养基组分为:硫胺素(B1)0.02mg/L、生物素(Bio)0.05mg/L、CaCO382g/L和乙酸钠4g/L.在最优培养基中,α-KG产量达到26.8g/L,提高了34%.α-酮戊二酸发酵过程动力学分析表明,采用最优培养基使发酵延滞期缩短6h,菌体比生长速率和α-KG比产物生成速率分别提高了45.4%和8.64%.The concentrations of thiamine, biotin, CaCO3 and sodium acetate in fermentation medium for α-ketoglutaric acid （α-KG） production by Torulopsis glabrata CCTCC M202019 were optimized using the response surface method. Among these factors, thiamine and CaCO3 played key role in α-KG production based on the results from full factorial designing. Then, the ranges of tiliamine and CaCO3 were estimated by the steepest ascent. The optimum fermentation medium composition for α-KG production was determined by the central composite design as follows： 0. 02 mg/L thiamine, 0. 05 mg/L biotin, 82 g/L CaCO3 and 4 g/L sodium acetate. With the optimum conditions, the yield of α-KG reached 26. 8 g/L, and was 34% higher than that in the control （ original medium）. The kinetic analysis indicated that the lag phase was 6 h shortened, and the specific rate of growth and α-KG production were increased by 45.4% and 8.64% , respectively, compared with that of the control. Fig 2, Tab 3, Ref 12

关 键 词：光滑球拟酵母 Α-酮戊二酸 响应面分析 动力学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]