非均相固定化酶促反应中外扩散抑制和化学抑制间的协同作用  

Co-Interaction Between Outside Diffusion Inhibition and Chemical Inhibition in the Reactions Catalyzed by Non-Homogeneous Phase Immobilized Enzymes

作　　者：邹晋[1] 邸进申[1] 

机构地区：[1]河北工业大学化工系,天津300130

出　　处：《河北工业大学学报》1997年第2期101-104,共4页Journal of Hebei University of Technology

基　　金：河北工业大学"50工程"资助

摘　　要：详细分析和讨论了外扩散抑制与化学抑制间的相互作用．结果表明：总有效系数η等于外扩散有效系数ηdif与化学抑制有效系数ηch之积，并且分别小于ηdif和ηch在外扩散抑制与竞争性、非竞争性或反竟争等不同化学抑制共存的反应体系中，底物浓度具有不同的影响；外扩散抑制与化学抑制间存在“抗激发互交作用”．从而对外扩散抑制与化学抑制共存的固定化酶促反应有了进一步的认识．The co-interaction between outside diffusion inhibition and chemical inhibition is analysed in detail. The total effective coefficient n equals the product of outside diffusion inhibition effective coefficient ηdifand chemical inhibition effective coefficient ηchand ηis less than ηdifand ηch respectively. There are different effects of the substrate concentration in this kind of reaction in which there are outside diffusion inhibition and competitive noncompetitive or uncompetitive chemical inhibition respectively. There is an antienergistic interaction in the reactions in which the two kinds of inhibitions exist. From tile results we have a better understanding for this kind of reactions catalyzed by immobilized enzymes in which there are outside diffusion inhibition and chemical inhibition.

关 键 词：固定化酶 扩散抑制 化学抑制 协同作用 酶促反应 

分 类 号：TQ033[化学工程] Q55[生物学—生物化学]