2-羧基苯偶氮若丹宁固相萃取光度法测定生物样品中的铅  

Study on Solid Phase Extraction and Spectrophotometric Determination of Lead in Biological Sample with 2-Carboxyphenylazo-Rodanine

作　　者：杨艳[1] 柴跃东[1] 李明[1] 史云东[1] 谢兢[1] 

机构地区：[1]玉溪师范学院化学与环境科学系,云南玉溪653100

出　　处：《食品科学》2008年第2期307-309,共3页Food Science

摘　　要：研究了2-羧基苯偶氮若丹宁(CPAR)与铅的显色反应,在pH4.0的盐酸-六次甲基四胺缓冲介质与乳化剂OP存在下,CPAR与铅反应生成2:1稳定络合物,可被Waters Sep-PakC18小柱固相萃取,用氮-氮二甲基甲酰氨(DMF)洗脱后于520nm处光度法测定,体系摩尔吸光系数ε为6.75×104L/mol·cm,铅含量在0.05～4.0μg/ml内符合比尔定律,本方法可用于生物样品中铅含量的测定,结果令人满意。The color reaction of lead with 2-carboxyphenylazo-rodanine （CPAR） was studied, In the presence ofpH4.0 buffer solution and emulsifier-OP medium, CPAR could react with lead（H） to form a stable 2：1 complex. This complex extracted by Waters Sep-Pak-C18 cartridge and eluted by DMF, its spectrophotometry determination results showed that the molar absorptivity is 6.75× 10^4 L/mol-cm at 520 nm. Beer＇s law was obeyed in range of 0.05～4 μg/m/. This method can be applied for the determination of lead in biological sample with satisfactory results.

关 键 词：2-羧基苯偶氮若丹宁 铅 固相萃取光度法 生物样品 

分 类 号：TS207.5[轻工技术与工程—食品科学]