胃镜下局部注射大蒜素对进展期胃癌细胞增殖及凋亡的影响  

Effect of Local Application of Allicin via Gastroscopy on Cell Proliferation and Apoptosis of Progressive Gastric Carcinoma

作　　者：张志栋[1] 李勇[1] 焦志凯[1] 赵雪峰[1] 范立侨[1] 赵群[1] 张立玮[1] 吴明利[1] 王顺平[1] 高阳[1] 

机构地区：[1]河北医科大学第四医院,石家庄050011

出　　处：《中国中西医结合杂志》2008年第2期108-110,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的探讨胃镜下大蒜素局部应用对进展期胃癌的影响及作用机制。方法进展期胃癌患者80例,胃镜及病理均证实为腺癌,分为两组;大蒜素组40例,术前48h胃镜下病变处局部注射大蒜素,对照组40例局部注射生理盐水;手术切除胃癌组织,采用美国Becton Dickinson公司产的FACS-420型流式细胞仪检测,观察胃癌组织周期(G0/G1期、S期、G2/M期)各时相百分比、细胞凋亡率、增殖指数(proliferation in-dex,PI),以及胃癌组织细胞凋亡相关基因(Fas、Bax、Bcl-2)蛋白表达。结果大蒜素组与对照组细胞凋亡率(%)分别为9.60±1.52、2.20±0.58,G0/G1期(%)分别为72.12±8.35、69.56±5.15,G2/M期(%)分别为9.54±3.20、13.20±3.05,PI值分别为27.80±8.35、30.40±5.15;两组比较,差异均有统计学意义(P<0.05,P<0.01);大蒜素组可增加凋亡促进基因Bax基因蛋白、凋亡始动基因Fas基因蛋白的表达(P<0.05,P<0.01),降低凋亡抑制基因Bcl-2基因蛋白的表达(P<0.05)。结论本项研究初步证明胃镜下局部注射大蒜素能够抑制进展期胃癌组织细胞生长、增殖,并促进胃癌细胞凋亡。Objective To study the effects of local application of allicin via gastroscopy on progressive gastric carcinoma, and to investigate its possible mechanisms. Methods Eighty patients with progressive gastric adenocarcinoma, whose diagnosis was confirmed by gastroscopy and pathological examination, were assigned to 2 groups, 40 in each group. Forty-eight hours before operation, allicin was infused via gastroscopy to the lesion region of patients in the allicin group, and normal saline was infused instead to those in the control group. The gastric carcinoma tissue gotten from gastrectomy was taken to determine the percentage of cells in various cell cycle phases （ G0/ G1, S and G2/M）, the cell apoptosis rate, proliferation index value and apoptosis related gene protein such as Fas, Bax and Bcl-2 by flow cytometry. Results In the allicin group, the cell apoptosis rate was 9. 60 ± 1.52%, the percentage of cell in G0/G1 phase was 72. 12 ± 8.35%, in G2/M phase 9. 54 ± 3.20%, and PI 27.80 ± 8.35, while in the control group, the corresponding data was 2. 20 ± 0. 58%, 69. 56 ± 5.15%, 13.20 ± 3.05%, and 30. 40 ±5.15, respectively, and significant difference in all the 4 indexes could be found between the two groups （P 〈0. 05, P 〈0. 01 ）. Moreover, allicin showed effects in up-regulating the protein expressions of apoptosis promoting gene Bax and apoptosis initiating gene Fas （P 〈0. 05, P 〈0. 01 ）, and down-regulating that of anti-apoptosis gene Bcl-2 （P 〈 0. 05 ）. Condusion Local application of allicin via gastroscopy can inhibit the cell and proliferation of progressive gastric carcinoma, and can also promote gastric carcinoma cell apoptosis.

关 键 词：大蒜素 胃镜 胃癌 细胞周期 凋亡 凋亡相关基因 

分 类 号：R735.2[医药卫生—肿瘤] R733.71[医药卫生—临床医学]