Flow-injection chemiluminescence determination of tryptophan using galangin-potassium permanganate-polyphosphoric acid system  

作　　者：Hui Chen Li Li Min Zhou Yong Jun Ma 

机构地区：[1]College of Chemistry and Chemical Engineering, Gansu Key Laboratory of Polymer Materials, Northwest Normal University, Lanzhou 730070, China

出　　处：《Chinese Chemical Letters》2008年第2期203-206,共4页中国化学快报（英文版）

摘　　要：A high sensitive flow-injection chemiluminescence （FI-CL） method for the determination of tryptophan has been developed. The method is based on the chemiluminescence reaction of galangin-potassium permanganate-tryptophan in polyphosphoric acid （PPA） media. Under the optimized conditions, tryptophan was determined in the range 0.05-10 μg/mL with the detection limit （3tr） of 5.0 × 10^-3 μg/mL. The relative standard deviation （RSD） was 1.0% for 11 replicate determinations of 1.0 μg/mL tryptophan. Three synthetic samples were determined selectively with recoveries in the range from 99.6% to 102.0% in the presence of other amino acids.A high sensitive flow-injection chemiluminescence （FI-CL） method for the determination of tryptophan has been developed. The method is based on the chemiluminescence reaction of galangin-potassium permanganate-tryptophan in polyphosphoric acid （PPA） media. Under the optimized conditions, tryptophan was determined in the range 0.05-10 μg/mL with the detection limit （3tr） of 5.0 × 10^-3 μg/mL. The relative standard deviation （RSD） was 1.0% for 11 replicate determinations of 1.0 μg/mL tryptophan. Three synthetic samples were determined selectively with recoveries in the range from 99.6% to 102.0% in the presence of other amino acids.

关 键 词：CHEMILUMINESCENCE FLOW-INJECTION GALANGIN Potassium permanganate TRYPTOPHAN 

分 类 号：TQ2[化学工程—有机化工]