全反式维甲酸提高激素非依赖性前列腺癌自杀基因治疗的旁观者效应  

All-trans Retinoic Acid Enhances Bystander Effect of Suicide-gene Therapy Against Androgen-unresponsive Prostate Cancer

作　　者：陈卫国[1] 严春寅[1] 侯建全[1] 温端改[1] 浦金贤[1] 王恒兵[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,江苏苏州215006

出　　处：《中华男科学杂志》2008年第2期122-125,共4页National Journal of Andrology

基　　金：江苏省科技发展计划基金(BS2003013)

摘　　要：目的:观察全反式维甲酸(ATRA)提高单纯疱疹病毒胸苷激酶(HSV-TK)/丙氧鸟苷(GCV)系统体内外抗激素非依赖性前列腺癌的旁观者效应。方法:应用四甲基偶氮唑蓝(MTT)法测定细胞存活率来反映ATRA处理前后HSV-TK/GCV系统治疗PC-3细胞的旁观者效应;荷前列腺癌裸鼠模型随机分为4组,观察各组移植瘤生长状态和组织病理学改变。结果:在达到明显旁观者效应时,HSV-TK/GCV需要TK+PC-3细胞数为50%,而ATRA联合HSV-TK/GCV需要TK+PC-3细胞数仅为30%,两者比较差异显著(P<0.05)。HSV-TK可以抑制移植瘤生长,但ATRA+HSV-TK抗前列腺癌发生显效可提前1周,而且效果更显著(P<0.05)。结论:ATRA可增强HSV-TK/GCV系统治疗激素非依赖性前列腺癌的旁观者效应。Objective： To investigate the enhancing effect of all-trans retinoic acid （ATRA） on the bystander effect of the herpes simplex virus thymidine kinase（HSV-TK）/ganciclovir（GCV） against androgen unresponsive prostate cancer. Methods： The bystander effect of the HSV-TK/GCV system was measured by methylthiazolyle tetrazolium （MTT） assay on PC-3 cells before and after ATRA treatment. The growth and the histopathology of transplant tumors were observed in 4 groups of nude mice with prostate cancer. Resuits ： ATRA augmented significantly the bystander effect of the HSV-TK/GCV system by reducing TK positive PC-3 cells from 50% to 30% （P 〈0.05）. HSV-TK showed an inhibiting effect, while ATRA with the HSV-TK/GCV system produced significant effect on prostate cancer 1 week earlier than the former （ P 〈 0.05 ）. Conclusion ： ATRA can argument the in vivo and in vitro bystander effect of the HSV-TK/GCV system in the treatment of androgen unresponsive prostate cancer. Natl J Androl, 2008, 14（2 ） ： 122-125

关 键 词：前列腺肿瘤 维甲酸 自杀基因 治疗 旁观者效应 

分 类 号：R737.25[医药卫生—肿瘤] R730.5[医药卫生—临床医学]