JNK通路在氯通道阻断剂抑制Aβ25-35诱导细胞凋亡中的作用  

The effect of JNK transduction pathway on Aβ_(25-35) induced and chloride channel blockers blocked cell apoptosis

作　　者：啜玉彩[1] 李源[2] 梁东明[2] 毕辉[3] 宋宏鲁[3] 龚卫琴[1] 

机构地区：[1]第四军医大学西京医院老年病科,陕西西安710033 [2]第四军医大学基础部生理学教研室 [3]第四军医大学西京医院眼科

出　　处：《中国老年学杂志》2008年第4期316-318,共3页Chinese Journal of Gerontology

基　　金：陕西省自然科学基金(2006C_212)

摘　　要：目的探讨JNK通路在氯通道阻断剂4,4'-diisothiocyano-2,2'-disulfonie acid stilbene(DIDS)及Phloretin在β淀粉样蛋白活性片段(Aβ,Aβ_(25-35)诱导PC12细胞凋亡过程中作用。方法采用MTT比色法、乳酸脱氢酶(lactate dehydrogen,LDH)活力检测、琼脂糖凝胶电泳法,比较正常对照组、Aβ_(25-35)损伤组和Aβ_(25-35)+Phloretin组、Aβ_(25-35)+DIDS组的PC12细胞存活与凋亡;Westem印迹法检测4组的JNK、P-JNK蛋白表达。结果10μmol/L Phloretin和50μmoL/L DIDS均能抑制Aβ_(25-35)诱导的PC12细胞凋亡,提高细胞存活率,减少LDH释放,降低P-JNK蛋白的水平。结论JNK的磷酸化水平下调,参与了氯通道阻断剂抑制的Aβ_(25-35)诱导的PC12细胞凋亡。Objective To investigate the effect of JNK transduction pathway on Aβ25-35 induced and chloride channel blockers 4, 4＇-diisothiocyano-2, 2＇-disulfonic acid stilbene （DIDS） and Phloretin blocked PC12 cell apoptosis. Methods PC12 cell survival and apoptosis rate in control, Aβ25-35, Aβ25-35 ＋ phloretin and Aβ25-35 ＋ DIDS groups were compared by MTY assay, released LDH assay, agarose gel erectrophoresis of DNA. The expressions of JNK and phosphorylated （P）-JNK protein were measured by Western blot assay. Results 10 μmol/L Phloretin or 50 μmol/L DIDS inhibited the apoptosis of PC12 cells induced by Aβ25-35 significantly, elevated cell survival rate, decreased release of LDH, and lowered P-JNK expressions （P 〈 0. 01 ）. Conclusions The suppression of P-JNK is involved in Aβ25-35 induced and chloride channel blockers blocked PC12 cell apoptosis.

关 键 词：Β淀粉样蛋白 细胞凋亡 JNK PHLORETIN DIDS 

分 类 号：R749.16[医药卫生—神经病学与精神病学]