肺缺血再灌注损伤中氧化应激与Fas/FasL系统的关系  

Relationship Between Oxidative Stress and Fas/FasL System During Ischemia Reperfusion of Lung in Rabbits

作　　者：郝卯林[1] 王万铁[1] 徐正祄[1] 王方岩[1] 张晓隆[1] 

机构地区：[1]温州医学院基础医学院病理生理学教研室,浙江省温州市325035

出　　处：《中国动脉硬化杂志》2007年第11期823-826,共4页Chinese Journal of Arteriosclerosis

基　　金：浙江省教育厅科研基金项目(20000670);温州市科技计划重点项目(Y2005A081)

摘　　要：目的探讨肺缺血再灌注损伤中氧化应激与Fas/FasL系统的关系。方法采用在体兔单侧肺缺血再灌注损伤模型,日本大耳白兔40只,随机分为对照组,缺血再灌注1h、3h和5h组,每组10只。对比观察各组血浆超氧化物歧化酶活性、丙二醛含量和一氧化氮水平,以及肺组织细胞凋亡指数及Fas/FasL mRNA定位表达。结果缺血再灌注组肺组织凋亡指数明显高于对照组(P<0.01);再灌注后Fas、FasL mRNA在肺血管内皮细胞、肺泡上皮细胞及支气管上皮细胞等呈阳性表达,明显强于对照组(P<0.01);丙二醛含量随再灌注时间延长逐渐增加(P<0.01),而超氧化物歧化酶活性与一氧化氮则随再灌注时间延长有所降低(P<0.01)。结论肺缺血再灌注损伤期间,氧化应激参与Fas/FasL系统的激活。Aim To investigate the relationship between oxidative stress and Fas/FasL system during lung ischemia reperfusion in rabbits.Methods Rabbit lung model of ischemia reperfusion injury was established in vivo.Forty rabbits were randomly divided into control group and ischemia reperfusion 1 h,3 h,and 5 h group,10 rabbits in each group.The activity of plasma superoxide dismutase,the content of plasma nitric oxide and the concentration of malondialdehyde,pneumocyte apoptosis index and express of Fas/FasL mRNA were measured respectively in different groups. Results Compared with control group, apoptosis index was significantly high （ P 〈 0.01）, Fas/FasL mRNA heavily expressed in lung vascular endothelial cells, alveolar and bronchial epithelial cells in ischemia reperfusion groups （ P 〈 0. 01 ）. The activity of superoxide dismutase and the content of nitric oxide showed a time-dependent decrease in ischemia reperfusion groups （ P 〈 0.01 ）, while the content of malondialdehyde was gradually increased with the time of reperfusion prolonging （ P 〈 0.01 ）. Conclusion Oxidative stress was associated with activation of Fas/FasL during lung ischemia reperfusion.

关 键 词：病理学与病理生理学 肺 缺血再灌注 凋亡 FAS/FASL 活性氧 一氧化氮 

分 类 号：R363[医药卫生—病理学]