肝细胞生长因子对四氯化碳损伤原代培养大鼠肝细胞的保护作用  

PROTECTION OF HEPATOCYTE GROWTH FACTOR AGAINST CARBON TETRACHLORIDE INJURY IN PRIMARY RAT HEPATOCYTE CULTURE

作　　者：邹原[1] 宫德正[1] 孙丽娟[1] 梅懋华[1] 

机构地区：[1]大连医科大学生理教研室

出　　处：《中国应用生理学杂志》1997年第3期228-230,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：本工作采用无血清原代培养大鼠肝细胞法，观察了重组人肝细胞生长因子（ｒ－ｈＨＧＦ）对四氯化碳（ＣＣｌ４）致大鼠肝细胞损伤的保护作用。结果表明，ｒ－ｈＨＧＦ对ＣＣｌ４染毒肝细胞有明显的保护作用。ｒ－ｈＨＧＦ保护组较ＣＣｌ４染毒组细胞存活率显著升高，细胞内丙氨酸转氨酶、钾离子漏出明显降低。结果提示，ｒ－ｈＨＧＦ可减轻ＣＣｌ４对肝细胞膜的损伤。The protective effect of recombinant human hepatocyte growth factor (r hHGF) against carbon tetrachloride (CCl 4) injury in cultured rat hepatocytes was investigated. It was found that r hHGF had cytoprotective effect on hepatocytes anainst CCl 4 poisoning. Pretreatment with r hHGF significantly increased the viability of cultured hepatocytes, reduced the leakage of intracellular alanine transaminase and potassium into the culture medium, as compared with the CCl 4 group. The results suggest that r hHGF may protect hepatocytes against CCl 4 injury through lessening the severity of membrane lesion,preventing intracellular alanine transaminase and potassium leakage and sustaining the integrity of hepatocyte membrane.

关 键 词：肝细胞生长因子 四氧化碳 肝细胞 原代培养 损伤 

分 类 号：R575.02[医药卫生—消化系统]