检测细胞代谢气体成分诊断肺癌的方法  

Lung cancer diagnosis method by detecting metabolic gases of lung cells

作　　者：郝燕[1] 王永清[2] 王悦[1] 陈星[1] 曹明富[1] 徐凤娟[1] 张伟民[2] 王平[1] 

机构地区：[1]浙江大学生物传感器国家专业实验室,生物医学工程教育部重点实验室,浙江杭州310027 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310016

出　　处：《浙江大学学报（工学版）》2008年第2期294-298,348,共6页Journal of Zhejiang University：Engineering Science

基　　金：国家自然科学基金资助项目(30570492);浙江省科技项目(021103007)

摘　　要：提出了一种在细胞水平进行肺癌病理气体检测和早期诊断的方法,分析了肺癌细胞新陈代谢产物中特征性挥发性有机气体成分(VOCs).采用固相微萃取-气相色谱(SPME-GC)气体检测装置来检测多种目标细胞的VOCs.选取13例肺癌病人手术切除后的肺癌组织和癌旁组织进行培养和电镜观察.通过对比不同类型肺癌细胞、肺癌细胞与同体正常肺部细胞、肺癌细胞与其他包括支气管上皮细胞、味蕾细胞、成骨细胞、脂肪粒细胞培养液中的VOCs,证明了肺癌细胞培养液中存在的特征性VOCs是肺癌细胞新陈代谢的产物,可以作为判定特定肺癌细胞的依据,为电子鼻呼吸诊断提供了重要依据.A new method for lung cancer pathology analysis and early diagnosis in cell level was presented to find the special volatile organic compounds （VOCs） in the metabolic product of lung cancer cells. A gas detection system using solid phase micro-extraction combined with gas chromatography （SPME-GC） was designed to detect the VOCs existing in the culture medium of several target cells. Lung tissues and their side tissues from 13 lung cancer patients were cultured and observed by electron microscopy, besides, dif- ferent kinds of cells including bronchial epithelial cells, taste bud cells, osteogenic cells and lipocytes from rats were cultured as control group. The results show that there are special VOCs in the culture medium of lung cancer cells, which are the metabolic product of lung cancer cells. The special VOCs can be viewed as biomarkers of lung cancer, so provide basis for electronic nose for non-invasive detection and diagnosis of lung cancer.

关 键 词：肺癌 特征性VOCs 呼吸气味诊断 无创诊断 电子鼻 

分 类 号：R734.2[医药卫生—肿瘤] R443.6[医药卫生—临床医学]