Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells  

作　　者：陈玮 赵涌 卞修武 

机构地区：[1]Department of Pathology Chongqing University of Medical Sciences [2]Institute of Pathology,Southwest Hospital,Third Military Medical University

出　　处：《Chinese Journal of Cancer Research》2008年第1期1-4,共4页中国癌症研究（英文版）

基　　金：the National High Tech R & D Program of China("863"project)(No.2002AA001010)

摘　　要：Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.

关 键 词：HPV IMMORTALIZATION TRANSCRIPTION p53 protein 

分 类 号：R96[医药卫生—药理学]