Study on the Quality of NILs of Wheat cv. Longfumai 3 Possessing HMW-GS Null and 1 Subunits  

作　　者：ZHANG Li-li ZHANG Yan-bin SONG Qing-jie ZHAO Hai-bin YU Hai-yang ZHANG Chun-liXINWen-li2 and XIAO Zhi-min2 XINWen-li XIAO Zhi-min 

机构地区：[1]College of Life and Environment Science, Harbin Normal University, Harbin 150025, P.R.China [2]Crop Breeding Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China

出　　处：《Agricultural Sciences in China》2008年第2期140-147,共8页中国农业科学（英文版）

基　　金：The study is financially supported by the National Natural Science Foundation of China(39770461).

摘　　要：To determine the genetic differences between high molecular weigh glutenin subunits （HMW-GS） null and 1. HMW-GS 1 was introduced into Longfumai 3 （N, 7＋8, 5＋10） by 5 consecutive backcrosses with biochemical marker assisted selection. The near isogenic lines （NILs） of HMW-GS null and 1 were obtained and planted in the experimental field of Crop Breeding Institute of Heilongjiang Academy of Agricultural Science in 2004 and 2005. The field experiments were designed using the method of two-line contrast arrangement with four replicates. The result of two years experiment showed that the statistic differences of quality parameters between Longfumai 3 with subunit 1 and with null were not significant in flour protein content, dry gluten content, and water absorption. However the gluten index,＇ Zeleny sedimentation, the ratio of sedimentation/dry gluten, the development time, stability, and the breakdown time in the NIL with 1 subunit were increased by 5.8% （P〈0.01）, 9.3% （P〈0.01）, 8.6% （P=0.01）, 127.3% （P〈0.01）, 79.2% （P〈0.01）, and 53.6% （P〈0.01）, and the ratio of wet gluten/dry gluten and the degree of softening were decreased by 1.7% （P = 0.05） and 16.5% （P = 0.13）, respectively. The impact of the HMW-GS 1 on the gluten strength was highly positive in NILs containing HMW-GS 5 ＋ 10, suggesting that HMW-GS 1 can be an indispensable subunit for breeding strong gluten wheat.To determine the genetic differences between high molecular weigh glutenin subunits （HMW-GS） null and 1. HMW-GS 1 was introduced into Longfumai 3 （N, 7＋8, 5＋10） by 5 consecutive backcrosses with biochemical marker assisted selection. The near isogenic lines （NILs） of HMW-GS null and 1 were obtained and planted in the experimental field of Crop Breeding Institute of Heilongjiang Academy of Agricultural Science in 2004 and 2005. The field experiments were designed using the method of two-line contrast arrangement with four replicates. The result of two years experiment showed that the statistic differences of quality parameters between Longfumai 3 with subunit 1 and with null were not significant in flour protein content, dry gluten content, and water absorption. However the gluten index,＇ Zeleny sedimentation, the ratio of sedimentation/dry gluten, the development time, stability, and the breakdown time in the NIL with 1 subunit were increased by 5.8% （P〈0.01）, 9.3% （P〈0.01）, 8.6% （P=0.01）, 127.3% （P〈0.01）, 79.2% （P〈0.01）, and 53.6% （P〈0.01）, and the ratio of wet gluten/dry gluten and the degree of softening were decreased by 1.7% （P = 0.05） and 16.5% （P = 0.13）, respectively. The impact of the HMW-GS 1 on the gluten strength was highly positive in NILs containing HMW-GS 5 ＋ 10, suggesting that HMW-GS 1 can be an indispensable subunit for breeding strong gluten wheat.

关 键 词：WHEAT QUALITY GLUTEN near-isogenic lines （NILs） 

分 类 号：S512.1[农业科学—作物学]