耐低温螺旋蓝细菌诱变株的诱变及特性研究  

Mutation and Characteristics of Low Temperature Resistant Mutants of Spirulina maxima

作　　者：韩岚[1] 汪育文[1] 李建宏[1] 刘海燕[1] 陈明[1] 翁永萍[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097

出　　处：《食品科学》2008年第3期350-354,共5页Food Science

基　　金：教育部留学归国人员基金项目;人事部留学回国人员择优资助项目

摘　　要：为了获得能适应较低生长温度的菌种,对极大螺旋蓝细菌进行紫外诱变,通过低温筛选获得了生理性状稳定的耐低温诱变株。其中两个诱变株YB01和YB02在10、15和20℃低温条件下生长速率显著高于出发藻株,诱变株的胡萝卜素和不饱和脂肪酸的含量也比出发藻株明显提高,是两株较理想的诱变株。对YB02株的抗低温生理进行研究发现,其光合放氧速率和体内积累的脯氨酸含量明显高于出发藻株。此外,对YB02进行了二次诱变,获得两株在低温条件下生长速率更快的诱变株YB0201和YB0202。To obtain low-temperature resistant mutant strains, Spirulina maxima was induced by ultraviolet irradiation as a mutagen. Low temperature resistant mutants were screened under low temperature culturing. The mutants YB01 and YB02 grew significantly faster than their parental strain under low temperature 10 ~C, 15 ~C or 20 ~C respectively. Furthermore, the contents of carotene and polyunsaturated fatty acids of the mutants were higher than their parental strain obviously. Investigation on physiological characteristics of mutant YB02 showed that photosynthetic oxygen evolution rate and proline content are higher than their parental strain. To improve mutant growth, the daughter mutant YB02 are also mutated twice. Two granddaughter mutants YB0201and YB0202 are also obtained. Their growth is faster than YB02.

关 键 词：极大螺旋蓝细菌 紫外诱变 低温 不饱和脂肪酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]