假肥大型肌营养不良患儿肌型肌酸激酶亚型的研究  

Creatine kinase isoform of normal and Duchenne dystrophic children

作　　者：赵昕[1] 赵振军[1] 韩冰[1] 叶贤坤[1] 毕春雷 李振宇 杨振华[1] 

机构地区：[1]北京医院检验科石家庄开发区医药研究所

出　　处：《中华医学检验杂志》1997年第3期153-155,共3页

摘　　要：目的探讨假肥大型肌营养不良（ＤＭＤ）患儿肌型肌酸激酶同工酶（ＣＫ－ＭＭ）亚型的变化在早期诊断和评价病情上的价值。方法采用不连续缓冲体系电泳法，在稳流和低压条件下电泳，将ＣＫ－ＭＭ亚型分离并荧光扫描。结果测定４０例ＤＭＤ患儿血清中ＣＫ－ＭＭ亚型，在３～７岁、～１０岁、～１４岁不同年龄组患儿中，ＭＭ３／ＭＭ１比值分别为０．６０８±０．３０９，０．７８１±０．４５２和１．１５８±０．５６５，而４０例对照组ＭＭ３／ＭＭ１比值为０．１５９±０．０６２。各年龄组ＤＭＤ患儿的ＣＫ－ＭＭ亚型改变与对照组比较，差异均有显著性（Ｐ＜０．０５），ＤＭＤ患儿各年龄组间ＣＫ－ＭＭ亚型改变差异也有显著性（Ｐ＜０．０５）。结论ＣＫ－ＭＭ亚型可为ＤＭＤ患儿的早期诊断和评价病情提供有价值的依据。Objective To study the changes of isoforms of CK MM in children with DMD for early diagnosis and evaluation. Methods A discontinuous buffer system was used. CK MM isoform was separated by electrophoresis, under constant current and low voltage, fluorescence scanning. Results Isoform of CK MM was determined in 40 children with DMD their MM 3/MM 1 ratio was 0.608±0.309, 0.781±0.452, 1.158±0.565 in 3～7, ～10, ～14 age groups respectively. The MM 3/MM 1 ratio of control group was 0.159±0.062. There was a statistically significant MM 3/MM 1 ratio in subjects when compared with control group and self differential age group. Conclusion The sutdy of isoforms provides useful information in early diagnosis and evaluation of children with DMD.

关 键 词：肌酸激酶 同工酶 肌营养不良 儿童 

分 类 号：R746.2[医药卫生—神经病学与精神病学]