牛磺酸对微囊化肝细胞生长的影响  

Influence of taurine on the growth of microencapsulated hepatocytes

作　　者：孙志杰[1,2] 郭昕[1] 宁小娟[3] 李双月[1,2] 吕国军[1,2] 于炜婷[1] 王为[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医学材料工程组,辽宁省大连市116023 [2]中国科学院研究生院,北京市100039 [3]江西农业大学理学院,江西省南昌市330045

出　　处：《中国组织工程研究与临床康复》2008年第6期1031-1034,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家“八六三”项目(2005CB522702);国家自然科学基金资助项目(20236040,30472102)~~

摘　　要：目的:微胶囊中存在一定程度的高渗透压胁迫,可造成细胞生长代谢减慢,为改善微囊化肝细胞的生长,尝试应用抗渗透压胁迫物质牛磺酸,观察其对微囊化肝细胞生长的影响。方法:实验于2006-07/08在中国科学院大连化学物理研究所生物医学材料工程实验室完成。将HepG2细胞悬液(非微囊化细胞)和微囊化HepG2分别接种于牛磺酸浓度为0,0.6,0.8和1.2mmol/L的MEM培养液中,在37℃体积分数为0.05的CO2中培养。MTT法测定非微囊化和微囊化HepG2细胞的生长活性,相差显微镜观察囊内细胞生长状态。结果:①非微囊化HepG2细胞:0.6,0.8和1.2mmol/L的牛磺酸对其生长无影响,吸光度值比较差异无显著意义(P>0.05)。②微囊化HepG2细胞:0.6,0.8mmol/L牛磺酸对微囊化肝细胞的生长亦无影响(P>0.05);但1.2mmol/L牛磺酸可以显著改善微囊化肝细胞的生长,其吸光度值高于0mmol/L牛磺酸组(P<0.05),并促进细胞的聚集。结论:1.2mmol/L牛磺酸可以改善微囊化肝细胞的生长并抵制微囊内的高渗透压胁迫对细胞的生长抑制作用。AIM： Hyper-osmotic stress may occur in the microcapsules, and reduce the metabolism of cells. This study was designed to investigate the influence of taurine, as an osmolarity substance, on the growth of microencapsulated hepatocytes. METHODS： The experiment was done in the Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics of the Chinese Academy of Sciences between July and August in 2006. Suspended HepG2 cells （control group） and microencapsulated HepG2 cells were inoculated into MEM mediums which contained 0, 0.6, 0.8 and 1.2 mmol/L taurine and cultured at 37 ℃ in an atmosphere with 0.05 volume fraction of CO2. Growth activities of suspended HepG2 cells and microencapsulated HepG2 cells were determined by MTT methods, and the growth of microencapsulated HepG2 cells was observed under phase-contrast microscopy. RESULTS：1.Taurine at 0.6, 0.8 and 1.2 mmol/L had insignificant effects on the growth of non-encapsulated HepG2 cells. There was no significant difference in the absorptance value （P 〉 0.05）.2.Taurine at 0.6 and 0,8 mmol/L did not alter the growth of microencapsulated cells （P 〉 0.05）, but taurine at 1.2 mmol/L increased the growth activities and prompted cell aggregation of microencapsulated HepG2 cells, The absorbtance value was also higher compared to that in 0 mmol/L taurine treatment （P 〈 0.05）. CONCLUSION： Taurine at 1.2 mmol/L can significantly facilitate the growth of microencapsulated HepG2 cells and can prevent the inhibition effects of hyper-osmotic stress on cellular growth.

关 键 词：微囊化 牛磺酸 渗透压胁迫 肝细胞 生长 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]