姜黄素对白血病细胞的细胞周期阻断与细胞周期蛋白的关系  

Relationship between Curcumin-induced cell cycle arrest and cyclins in leukemia cells

作　　者：林琦[1] 吴丽贤[1] 许建华[2] 黄秀旺[1] 张昆仲[1] 温彩霞[1] 陈元仲 

机构地区：[1]福建医科大学药理系,福建福州350004 [2]福建医科大学临床药理研究所,福建福州350004 [3]福建省血液研究所,福建福州350004

出　　处：《世界肿瘤杂志》2008年第1期9-12,共4页Tumour Journal of the World

基　　金：国家自然科学基金（No30171158,30472187）资助：福建省资助省属高校项目（N02005K048）资助

摘　　要：目的研究姜黄素（Cur）对K562细胞和HL-60细胞的细胞周期阻断与细胞周期蛋白的关系。方法用流式细胞光度术对K562细胞和HL-60细胞进行细胞周期检测，用蛋白免疫印迹检测细胞周期蛋白水平。结果姜黄素0—10mg／L作用24h可将K562细胞和HL-60细胞阻滞于G0／G1期，该期细胞比例增加；阻断细胞从S期向G2／M期转化，G2／M期细胞比例减少；姜黄素作用24h减少K562细胞和HL-60细胞Cyclin D1、Cyclin B1的蛋白水平，但几乎不影响K562细胞和HL-60细胞Cyclin A的蛋白水平。结论姜黄素扰乱K562细胞和HL-60细胞的细胞周期进程与Cyclin D1和Cyclin B1的蛋白水平减少有一定关系。AIM To study the effects of curcumin on cell cycle progression in k562 and HL-60 cell lines and determine the relationship of the effect to cyclins. Methods Flow cytometry was used to examine curcumin-induced cell cycle arrest, and western blot was used to analyze the levels of cyclins in curcumin-treating cells. Results An exposure of k562 and HL-60 cells to Cur 0-10μg/ml for 24h produced a dose dependent increase in G0/G1 phase cells from 32.6% and 36.8% to 47.6 % and 57.9%, respectively; G2/M phase cells were decreased from 11.6% and 2.8% to 0% and 0%, respectively. Consistent with this effects, the abundance of Cyclin D1, Cyclin B1 was deregulated in a dose dependent way, while the levels of Cyclin A were not changed. Conclusions The cell cycle arrest induced in k562 and HL-60 cells by curcumin was correlated with deregulation of the abundance of Cyclin D1 and Cyclin B1.

关 键 词：姜黄素 细胞周期阻断 细胞周期蛋白 CYCLIN D1 CYCLIN A CYCLIN B1 

分 类 号：R733.7[医药卫生—肿瘤] R73-34[医药卫生—临床医学]