薄层色谱法快速分析乳果糖  

Rapid Determination of Lactulose by a Thin-layer Chromatography Method

作　　者：刘芳[1] 杨瑞金[2] 张文斌[1] 张萃荟[1] 费颖[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2008年第1期119-123,共5页Food and Fermentation Industries

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA10Z336)

摘　　要：以硅胶G板为固定相,正丁醇-乙醇-水(体积比5:3:2)为流动相,研究并建立了乳果糖的薄层层析快速分析方法。经过上行展开,苯胺-二苯胺-磷酸显色,根据葡萄糖、半乳糖、果糖、乳糖、乳果糖各斑点的R_f值和各自的斑点颜色定性。运用飞点扫描仪扫描乳果糖斑点的峰面积,并依据建立的标准曲线计算酶反应液中的乳果糖含量。在所选择的实验条件下,乳果糖的检测限为2μg,在2～30μg范围内,其斑点的峰面积与检测量具有一定的正相关性。用定量添加标准品的方法研究薄层扫描的准确度,其相对标准偏差为3.41%。此方法设备简单、操作成本低,可以应用于实验室的初期研究阶段。A thin-layer chromatographic method for lactulose determination was established,in which the mobile phase was butanol-alcohol-water （5：3：2,v/v/v）.Lactulose,lactose,glucose,galaetose and fructose were developed by ascending technique on the silica gel G plate,and visualized by aniline—diphenylamine—phosphoric acid.The Rf value of each saccharide was different and the color of the spots was characterized.The calibration curve was linear in the range of 2—30μg and the detection limit was 2μg.To determine the chromatographic peak areas of lactulose,mottles with thin—layer scanner in the sawtooth scanning mode was described.The detective wavelength was 526 nm.The concentration of laetulose was calculated according to the calibration curve.Accuracy was certified by external standard method.Relative standard deviation （RSD） was 3.41%.This method is simple,inexpensive and do not need large-volume unit.It has been applied to the batching of lactulose,which could provide reference for reaction conditions.

关 键 词：乳果糖 薄层色谱 薄层扫描 定量添加标准品法 

分 类 号：TS247[轻工技术与工程—制糖工程]