p57^(kip2)在人肝细胞癌中杂和性缺失研究  

Loss of heterozygosity of p57^(kip2) in human hepatocellular carcinoma

作　　者：孙江华[1] 孔萌萌[2] 章宗籍[3] 申丽娟[3] 

机构地区：[1]武警湖北总队后勤部卫生处,湖北武汉430070 [2]同济大学生命科学与技术学院,上海200092 [3]昆明医学院病理教研室,云南昆明650031

出　　处：《武警医学院学报》2008年第2期103-105,共3页Acta Academiae Medicinae CPAPF

摘　　要：【目的】研究p57kip2基因在人肝细胞癌(hepatocellular carcinoma,HCC)不同阶段的杂合性缺失(loss of heterozygosity,LOH),以探讨HCC发生的分子生物学机制。【方法】运用PCR聚丙烯酰胺凝胶电泳-银染法对30例肝癌组织p57kip2基因位点上2个微卫星位点(TH01和D11S2359)进行LOH的检测。【结果】30例肝癌组织中有9例至少在1个微卫星位点(30.0%)表现出LOH,且分化好肝癌组织LOH率明显低于和分化差肝癌组织。【结论】p57kip2基因位点频繁的LOH在HCC发生中起重要作用。【Objective】To study the loss of heterozygosity（LOH）of p57kip2 in various stages of human hepatocellular carcinoma（HCC） in order to explore the molecular mechanism of hepatocarcinoma.【Methods】LOH of two microsatellite loci（TH01 and D11S2359）were detected in the p57kip2 gene site by using PCR-polyacrylamide gel electrophoresis-silver staining method.【Results】LOH was identifided at least in 9 cases out of 30（30.0%）on one microsatellite loci.The rate of LOH in well differentiated HCC tissues was significantly higher than that of poor differentiated HCC tissues.【Conclusions】The frequent LOH of p57kip2 gene plays an important role in the development of HCC.

关 键 词：肝癌 P57KIP2 杂合性缺失 

分 类 号：R735.7[医药卫生—肿瘤]