紫外线与氯化锂复合诱变选育L-组氨酸产生菌  

Mutation Screening of L-histidine Producing Strain by UV and LiCl

作　　者：邱雁临[1] 梁亮[1] 汪亮[1] 

机构地区：[1]工业微生物湖北省重点实验室,湖北工业大学生物工程学院,湖北武汉430068

出　　处：《现代食品科技》2008年第3期217-219,共3页Modern Food Science and Technology

基　　金：工业微生物湖北省重点实验室开放基金资助项目(工大科[2004]4号文)

摘　　要：以一株谷氨酸棒杆菌(Corynebacterium glutamicum)S6为出发菌株,利用氯化锂、紫外线进行诱变,通过实验证明氯化锂诱变剂量在1.2%时致死率达到82.4%,紫外线在照射30s时致死率达到81.8%。利用氯化锂诱变谷氨酸棒杆菌S6,所得菌株D3的L-组氨酸产量为243mg/L,比出发菌株提高10.5%;以紫外线做诱变S6,所得高产菌株U1产量256mg/L,比出发菌株提高8.5%;以紫外线、氯化锂复合诱变S6,所得菌株N1产量为251mg/L,比出发菌株提高13.6%,结果显示,经紫外线与氯化锂复合诱变后的菌株N1产L-组氨酸的产量最高,比氯化锂诱变后的菌株产L-组氨酸量提高3.1%,比紫外线诱变后的菌株产L-组氨酸量提高5.1%。A L-histidine producing strain was mutated and screened from Corynebacterium glutamicum S6 by LiCl and UV. Results showed that the UV-induced lethality was 81.8% with the irradiating time of 30s, while the LiCl-induced lethality was 82.4% with the LiCl concentration of 1.2%. Using three mutants, D3 by LiCl, U1 by UV, and N1 multiple mutated by UV and LiCl, the L-histidine yields reached 256mg/L, 243mg/L and 251 mg/L, respectively, which were 8.5%, 10.5% , and 13.6% respectively higher than that by the original strain S6.

关 键 词：紫外线 氯化锂 复合诱变 L-组氨酸 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]