应用流式细胞术对啤酒酵母在含油酸培养基中生长的定量研究  

Quantitative studies on the growth of Saccharomyces cerevisiae in oleic acid medium with flow cytometry

作　　者：张波[1] 陈君 卢启威 岳慧琴[2] 张丰德[2] 

机构地区：[1]广州海拓生物材料科技有限公司 [2]南开大学生命科学学院,天津300071

出　　处：《现代仪器》2008年第2期17-20,16,共5页Modern Instruments

摘　　要：本研究采用流式细胞术测定啤酒酵母中的绿色荧光蛋白的表达量和细胞的存活度,提出一种可以用于监测啤酒酵母生长的快捷方法,筛选出较佳细胞培养方案,测定酵母细胞在不同含油酸培养基中的死亡速度常数。Saccharomyces cerevisiae has been utilized to produce lipid-derived compounds and biopolymers, such as polyhydroxyalkanoates （PHA）. But these researches in S. cerevisiae are hampered by the fact that growth on fatty acid and microbody induction are often poor. The single-cell variability and the green fluorescent protein （GFP） expression of S. cerevisiae were measured by using flow cytometry technology. A fast way to examine S. cerevisiae growth was established and a better cultivation strategy was screened. The death rate constants of yeast ceils in different oleic acid media were calculated.

关 键 词：流式细胞术 啤酒酵母 油酸 存活度 绿色荧光蛋白 死亡速度常数 

分 类 号：Q2[生物学—细胞生物学] TS262.5[轻工技术与工程—发酵工程]