胃癌P53基因突变和蛋白表达的研究  

A STUDY OF P53 GENE MUTATION AND PROTEIN EXPRESSION IN HUNAN GASTRIC CANCER

作　　者：刘孟珉[1,2,3] 余龙[1,2,3] 潘星华[1,2,3] 叶天星[1,2,3] 孔宪涛 赵寿元[1,2,3] 

机构地区：[1]上海第二军医大学附属长征医院实验科 [2]复旦大学遗传学研究所 [3]第二军医大学生物学教研室

出　　处：《癌变．畸变．突变》1997年第5期271-274,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：为了解中国人胃癌Ｐ５３基因突变情况，应用银染聚合酶链反应—单链构象多态（ＰＣＲ—ＳＳＣＰ）技术对Ｐ５３基因第５、６、７、８外显子进行检测。对来自癌组织ＤＮＡ和癌旁正常组织ＤＮＡ的ＰＣＲ—ＳＳＣＰ电泳带迁移作对比分析，发现３７例胃癌中，１１例胃癌样品电泳带迁移异常，依据ＤＮＡ单链构象与分子电泳迁移的关系，研究结果表明：胃癌Ｐ５３基因５—８外显子的突变率为３０％。微波处理ＡＢＣ法检测突变型Ｐ５３蛋白表达，１７例为阳性，阳性率为４６％。这些资料提示：导致Ｐ５３蛋白异常表达的Ｐ５３基因突变是一种常见的分子结构改变，可能在胃癌的发生和发展中起着重要的作用。Silver staining PCR-SSCP method was used to detected the mutation of p53 gene exon5,6,7 and 8.Mobility of PCR amplification product from tumor tissue DNA was compared with that of para-cancer normal tissue DNA by PCR-SSCP .Mobility shife were observed in 11 out of 37 cases of gastric cancer.The date on relationship between mobility and single strand conformation showed that the mutation rate in exon 5,6,7 and 8 from these cancer cases was 30%.The mutant p53 protein was detected by microwave oven treatment and ABC immunohistochemistry,its positive nuclear staining was observed in 17 out of 37 cases of gastric cancer (46%).It is suggested that mutation of p53 gene,which result in abnormal protein accumulation,was a frequent molecular structural change and may have importance in carcinogenesis and progression of gastric cancer.

关 键 词：胃肿瘤 癌 P53基因 突变 蛋白 表达 

分 类 号：R735.202[医药卫生—肿瘤]