三氧化二砷诱导A549细胞凋亡及对survivin、cyclin D1表达的影响  

Effect of arsenic trioxide on inducing apoptosis of A549 cells and gene expression of survivin and cyclin D1

作　　者：曹琦[1] 郭红荣[1] 韦国桢[1] 马玲娣[1] 关乃富[1] 李世荣[1] 

机构地区：[1]南京医科大学附属常州第二人民医院,江苏常州213003

出　　处：《现代肿瘤医学》2008年第4期521-525,共5页Journal of Modern Oncology

摘　　要：目的:了解三氧化二砷对肺腺癌A549细胞株细胞增殖、细胞凋亡以及survivin、cylin D1基因表达的影响。方法:分别以不同浓度的三氧化二砷作用于A549细胞,作用不同时间对细胞增殖活性、细胞凋亡及细胞survivin、cylin D1基因表达的变化进行检测。结果:稍高浓度(>2μmol/L)的三氧化二砷可抑制A549细胞增殖,并有剂量和时间依赖性。逆转录聚合酶链式反应(RT-PCR)检测survivin、cylin D1基因转录水平和细胞爬片免疫组化检测survivin、cyclin D1基因蛋白表达的相对强度,发现三氧化二砷作用组survivin和cyclin D1基因的表达与对照组相比明显减低(P<0.01)。结论:三氧化二砷可有效抑制A549细胞增殖,诱导A549细胞凋亡,与作用剂量和作用时间呈正相关,这一过程可能与三氧化二砷抑制survivin、cyclin D1表达有关。Objective ：To investigat the effect of arsenic trioxide on cell proliferation,cell apoptosis and survivin, cyclin D1 gene expression in lung adenocarcinoma A549 cells. Methods：After A549 were treated with arsenic trioxide in different doses and with different time, MTT was used to detect the proliferation of A549 ceils. The Annexin V - FITC/PI double staining analysis by flow cytometry was used to evaluate apoptosis rate. Survivin ,cyclin D1 gene expression was detected by RT - PCR and immunohistochemesitry. Results： Arsenic trioxide could inhibit cell proliferation and induce cell apoptosis in A549 cells ,the apoptotic rate was dose depedent and time dependent. The survivin and cyclin D1 gene expression was down regulated in arsenic trioxide group compared with the control. Conclusion： Arsenic trioxide can effectively inhibit cell proliferatioin and induce apoptosis in lung adenocarcinoma A549 cells,this may result from its actioin of reduce survivin and gene expression.

关 键 词：肺腺癌 三氧化二砷 细胞凋亡 SURVIVIN CYCLIN D1 

分 类 号：R73-3[医药卫生—肿瘤] R734.2[医药卫生—临床医学]