非同位素PCR-SSCP-EB染色技术检测头颈部鳞癌p53基因点突变  

Detection of Point Mutation of p53 Gene in Head and Neck Squamous Cell Carcinoma by Non Isotopic PCR SSCP

作　　者：王建广[1,2,3,4] 黄洪章[1,2,3,4] 李金荣[1,2,3,4] 王刚 周刚[1,2,3,4] 黄玉屏 鲁德银[1,2,3,4] 侯炜 

机构地区：[1]湖北医科大学口腔医学院口腔颌面外科 [2]中山医科大学附属孙逸仙纪念医院 [3]上海铁路中心医院口腔科 [4]湖北医科大学病毒所

出　　处：《华西口腔医学杂志》1997年第3期215-217,共3页West China Journal of Stomatology

摘　　要：应用非同位素ＰＣＲ－ＳＳＣＰ－ＥＢ染色技术检测新鲜头颈部鳞癌组织中ｐ５３基因点突变。结果显示１３例标本中７例有异常电泳带，其中位于第５外显子者１例，位于第６，７，８外显子者各２例，说明头颈部鳞癌的发生与ｐ５３基因突变有密切关系。与传统的同位素ＰＣＲ－ＳＳＣＰ相比，非同位素ＰＣＲ－ＳＳＣＰ－ＥＢ染色技术是一种更简便、安全、快速、有效的检测基因点突变的方法，适用于大量标本基因点突变的筛选性检测。Non isotopic PCR SSCP technique was used to detect point mutation of p53 gene in fresh tissue taken from head and neck squamous cell carcinoma. The abnormal band of the single stranded DNA (ssDNA) was identified in 7 of 13 cases. The results showed that point mutation occured in the related DNA fragments. The point mutation of exon 5 was found in one case and that of exon 6,7,8 in two respectively. These results indicated that the p53 gene mutation was closely correlated with the development of head and neck squamous cell carcinoma. Compared with the traditional isotopic PCR SSCP technique, the non isotopic PCR SSCP one is a kind of simpler, safer, quicker and more effective way to detect the mutation of p53 tumor suppressor gene. It is especially useful in the screening of point mutation when handling a large number of samples.

关 键 词：PCR P53基因 基因突变 头颈部肿瘤 

分 类 号：R739.910.2[医药卫生—肿瘤]