产植酸酶青霉菌株的诱变研究  

STUDIES ON THE INDUCED MUTATION OF A PENICILLIUM STRAIN PRODUCING PHYTASE

作　　者：白玉明[1] 陈开明[1] 孟钊红 邓菊花 褚西宁[1] 

机构地区：[1]山西大学生命科学系

出　　处：《山西大学学报（自然科学版）》1997年第4期416-419,共4页Journal of Shanxi University(Natural Science Edition)

摘　　要：对产植酸酶的青霉菌株Ａ２进行了诱变研究。分别以ＨＮＯ２－ＵＶ和ＵＶ－５Ｂｕ复合处理，进行了二轮诱变筛选后，得到高于Ａ２５０％以上的变异株６株，其中Ａ２－ＵＶ５９－２０株的酶活达０．８１２±０．０１９ｕ／ｍｌ，为出发菌株Ａ２的二倍多。A wild Penicillium strain A 2 producing phytase was mutated for obtaining the enzymatic activity as high as possible. After two cycles of the complex mutation by using HNO 2 UV and UV-5BrU as well as the routine screening procedures,6 mutants that the enzyme activity was increased 50% more than that of A 2 were obtained.Among them,the best one is A 2-U59-20 which activity is more than 0.182±0.02U/ml,as two times as that of A 2.

关 键 词：植酸酶 青霉菌株 诱变育种 紫外辐射 产酶能力 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q939.970.3[生物学—微生物学]