Photosensitized damage to telomere overhang and telomerase RNA by riboflavin  

作　　者：Yuxia Liu Fuqiang Du Weizhen Lin Tiecheng Tu Wenxin Li Nianyun Lin 

机构地区：[1]Key Laboratory of Nuclear Analysis Techniques, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

出　　处：《Progress in Natural Science:Materials International》2008年第4期401-404,共4页自然科学进展·国际材料（英文版）

基　　金：National Natural Science Foundation of China（Grant No.20133020） ;the Photo Science and Technology Research Foundation of Science and Technology Committee（022261014）of Shanghai,China.

摘　　要：By ESR spin elimination and photocleavage assay, the mechanisms of one-electron oxidation damage of oligonucleotides by excited triplet state of riboflavin （Rb） have been elucidated. The results demonstrate that Rb, an endogenous photosensitizer, is capable of cleav- ing single-stranded telomeric overhang and the template region of telomerase RNA under UVA irradiation, resulting in blocking of reverse transcription of telomeric DNA which leads to the apoptosis of cancer cells ultimately. ~ 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.By ESR spin elimination and photocleavage assay, the mechanisms of one-electron oxidation damage of oligonucleotides by excited triplet state of riboflavin (Rb) have been elucidated. The results demonstrate that Rb, an endogenous photosensitizer, is capable of cleav- ing single-stranded telomeric overhang and the template region of telomerase RNA under UVA irradiation, resulting in blocking of reverse transcription of telomeric DNA which leads to the apoptosis of cancer cells ultimately.

关 键 词：TELOMERE TELOMERASE RIBOFLAVIN ESR spin elimination Excited triplet state 

分 类 号：Q522[生物学—生物化学]