激光免疫疗法对小鼠H22肝癌的长期抗瘤效应  

Long-term Anti-cancer Effect on H22 Liver Cancer after Laser Photo-immuno-therapy

作　　者：曾超英[1] 罗芳洪[1] 黄萍[1] 

机构地区：[1]厦门大学医学院抗癌研究中心,厦门市361005

出　　处：《中国激光医学杂志》2007年第1期1-3,共3页Chinese Journal of Laser Medicine & Surgery

基　　金：福建省重点科技计划项目(2001Z048)

摘　　要：目的观察激光免疫疗法对小鼠H22肝癌的抗瘤效应。方法昆明种小鼠240只,随机分为4组,每组60只。光动力学组:小鼠腹腔内注射HpD10 mg／kg后48 h行激光照射(功率320 W,时间400 s,能量130 J);激光免疫组:处理同前组,另于照光前12 h小鼠瘤内及瘤周注射糖基化氨基葡糖(GC) 0．2 ml／只;单纯GC组:仅于瘤内及瘤周注射GC;对照组:不做任何处理。比较前3个实验组的抑瘤率、生存率。结果3实验组小鼠均有显著抑瘤效果,激光免疫组的抑瘤率、生存率较单纯免疫佐剂治疗组和光动力学治疗组有显著提高,激光免疫组23．3％肿瘤治愈小鼠长期存活。结论激光免疫疗法对小鼠H22肝癌显示出长期抗瘤效应,该疗法可能成为一种长期有效的综合治疗肿瘤新方法。Objective To study the long-term anti-cancer effect on H22 liver cancer after laser photo-immunotherapy in mice. Methods Anti-cancer effect, tumor inhibition rate, survival rate and pathology were observed respectively in immunoadjuvant（ glycated chitosan）, photodynamic therapy （PDT） and laser photo-immunotherapy （ PDT ＋ glycated chitosan ） groups, as well as in control group（ n =60 in each group）. Results Immunoadjuvant, PDT and laser photo-immunotherapy could inhibit the tumor growth rate effectively compared to the contrast group. The tumor inhibition rate of laser photo-immunotherapy group was 60. 4% which was significantly higher than the immunoadjuvant and PDT groups. 23. 3% of mice of laser photo-immunotherapy group were cured and long - term survivable. Conclusions Laser photo-immunotherapy demonstrates long-term anti-cancer effect on H22 liver cancer which indicates that laser photo-immunotherapy maybe a novel modality for cancer treatment.

关 键 词：激光免疫疗法 免疫佐剂 抗瘤效应 

分 类 号：R735.7[医药卫生—肿瘤]