D-氨基葡萄糖对胶质瘤细胞生长特性影响研究  

Investigation of Effects of D-Glucosamine on Growth of Neurogliocytoma Cells

作　　者：张巍[1] 唐善虎[2] 秦文玲[1] 孟丽[2] 

机构地区：[1]成都理工大学,四川成都610059 [2]西南民族大学,四川成都610041

出　　处：《现代临床医学》2008年第2期83-85,共3页Journal of Modern Clinical Medicine

基　　金：四川省应用基础项目;课题计划编号:03JY029-039-1

摘　　要：目的:研究D-氨基葡萄糖对胶质瘤细胞的生长抑制作用,并探讨其作用机理。方法:采用MTT法筛选D-氨基葡萄糖最佳浓度,应用流式细胞仪检测D-氨基葡萄糖对细胞周期的影响。结果:D-氨基葡萄糖能明显抑制胶质瘤细胞生长,并且呈现一定的剂量依赖性。3.5 mg.L-1D-氨基葡萄糖表现出抑制率高峰,为21.4%。经D-氨基葡萄糖作用后细胞在G2-M期的比例增加,说明细胞停滞在G2-M期,但是未出现凋亡峰。结论:D-氨基葡萄糖能抑制人胶质瘤细胞的生长,其作用机理是使细胞生长停滞在G2-M期。Objective：To study the effects of D-glucosamine on the growth inhibition and induction of apoptosis in the neurogliocytoma cells in vitro.Methods：Cell proliferation was examined by MTT essay to optimize D-glucosamine concentrations.Cell cycles were detected based on cell DNA contents by flow cytometer（FCA）.Results：D-glucosamine had significant inhibition on the growth of neurogliocytoma cells in vitro.The concentration of D-glucosamine was 3.5 mg·L-1,and had maximal inhibition effects,the inhibition ratio was 21.4%,respectively.FCA analysis of U251 cells treated with D-glucosamine showed higher percentages in G2-M stage.Conclusion：D-glucosamine can restrain the growth of neurogliocytoma cells in vitro.It is postulated that growth retardation occurs at the G2-M stage.

关 键 词：D-氨基葡萄糖 胶质瘤 抗癌作用 体外培养 

分 类 号：R73-34[医药卫生—肿瘤]