骨髓间充质干细胞移植后在mdx鼠体内的分布研究  

Distribution of mesenchymal stem cells in mdx mice after transplantation

作　　者：卢锡林[1] 姚晓黎[1] 冯善伟[2] 于美娟[3] 陈松林[1] 张为西[1] 张成[1] 

机构地区：[1]中山大学附属第一医院神经科,广东广州510080 [2]中山大学干细胞与组织工程中心,广东广州510080 [3]广州医学院附属第二医院神经科,广东广州510260

出　　处：《中国病理生理杂志》2008年第4期763-766,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30370510,No30170337);广东省博士启动基金资助项目(No5300783;No04300353);广东省科技计划资助项目(No2006B36003003)

摘　　要：目的:研究骨髓间质干细胞移植治疗Duchenne型肌营养不良鼠(mdx鼠)后体内各主要器官的分布状况。方法:取第5代SD大鼠骨髓间质干细胞,应用[3H]-TdR标记后以2×107cells/只细胞尾静脉移植到经7Gyγ射线预处理后的7-9周龄mdx鼠体内,分别于移植后24h、48h、4周、8周和16周测定血液、肺、肝、骨髓、骨骼肌及心肌的放射性分布计数。结果:经7Gyγ射线放疗预处理的mdx鼠,在尾静脉移植骨髓干细胞后,24h肺的放射性分布计数最高,为36.70±3.04;48h肝的放射性分布计数最高,为36.74±3.28;骨髓分布计数随移植时间延长增多,2周时达到高峰,计数为38.43±4.99,以后逐渐下降,但4月时仍明显高于其它组织器官,计数为13.45±1.37;骨骼肌、心肌的放射性分布计数随移植时间延长明显增高,16周时分别达到4.79±0.94和9.55±1.53。结论:在MSCs移植后早期,MSCs主要分布于肺、肝等血流丰富的器官,随移植时间延长逐渐归巢到骨髓定居,2周时达到高峰,此后逐渐向损伤器官如骨骼肌、心肌等定向迁移,并且随时间延长分布增加,从而为MSCs定居于肌组织并且分化为肌细胞提供了证据。AIM： To investigate the distribution of mesenchymal stem cells in mdx mice after transplantation. METHODS ： P5 mesenchymal stem cells （MSCs） of SD rats labeled with [^3 H ] - TdB were injected intravenously into the mdx mice preconditioned with 7 Gy T ray. The mice were killed at 24 h, 48 h, 2 weeks, 1 month, 2 months, 4 months after transplantation of MSCs. Blood, lung, liver, bone marrow, heart, and skeletal muscle were collected, then the irradiated quantity was detected to calculate tissue specific localization account using scintillascope. RESULTS： Specific localization account in lung was the highest at 24 h. At 48 h liver was the highest. After transplantation the account of bone marrow increased and at 2 weeks reached the highest, then decreased as time going but was still higher than that of other organs. The account of skeletal muscle and heart also increased. CONCLUSION： At early time after transplantation, the MSCs labeled by [ ^3H] -TdR mainly distribute in lung and liver, then homing to bone marrow increasingly and the account is the highest at 2 weeks. MSCs migrate to injured organs, such as skeletal muscle and heart. The migration suggests that MSCs can settle down in muscles and provide evidence for MSCs to differentiate into myocytes.

关 键 词：骨髓干细胞移植 MDX鼠 

分 类 号：R746.2[医药卫生—神经病学与精神病学]