高效液相色谱法测定苦瓜啤酒中的亮蓝  

Detection of Brilliant Blue Content in Bitter Gourd Beer by HPLC

作　　者：李祝[1] 肖洋 

机构地区：[1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025 [2]贵州省产品质量检验检测院,贵州贵阳550003

出　　处：《酿酒科技》2008年第4期112-113,共2页Liquor-Making Science & Technology

摘　　要：探索利用高效液相色谱法快速测定啤酒中亮蓝的方法。样品经简单处理,在627nm波长下,以甲醇∶乙酸铵溶液(0.02mol/L)为45∶55为流动相,进行高效液相色谱分析,以保留时间定性,与标准比较定量。结果表明,其回收率为94.0%～98.3%,最低检出浓度为0.01mg/kg;该方法简便、快速、灵敏度高、准确可靠。Thempid measurement of brilliant blue content in bitter gourd beer by HPLC was investigated and the relative procedures were as follows： simple treatment of the samples, and then detected under 627 nm wavelength with moile phase as methyl alcohol ： acetic acid ammonium （0.02 mol/L）=45：55. The results showed that the recovery rate was between 94.0 %-98.3 % and the minimum detection limit was 0.01 mg/kg. Such method was simple and convenient and reliable with high sensitivity.

关 键 词：检测 高效液相色谱法 苦瓜啤酒 亮蓝 

分 类 号：TS262.5[轻工技术与工程—发酵工程]