鲟鱼原肌球蛋白的提取、结晶与电镜观察  

Purification Crysttalization and Electromicroscopic Observation of Tropomyosin of Sturgeon

作　　者：郭春生[1] 徐娉婷[1] 姚恒标 徐海成 

机构地区：[1]哈尔滨师范大学生物系

出　　处：《哈尔滨师范大学自然科学学报》1990年第3期91-95,共5页Natural Science Journal of Harbin Normal University

基　　金：校科研基金;校教学实践科的资助

摘　　要：本文对黑龙江省特产的软骨硬鳞鱼、鲟形目的重要代表动物鲟鱼的原肌球蛋白(Tropomyosin以下用TM表示)进行了提取、纯化和结晶,并对原肌球蛋白晶体做了光学显微镜和电子显微镜观察。在光镜下可见到原肌球蛋白或聚或散的针状结晶。在电镜下可看到鲟鱼原肌球蛋白晶体具有清晰的、明暗相间的原肌球蛋白所特有的特征性横纹周期,在横纹主周期另有若干横纹细节。Tropomyosin (TM) was isolated and purified from the body muscle of sturgeon [Acipenser schrencki (Brandt)]. Tropomyosin of sturgeon was crystalized in the presence of 0.05M MgC1_2— 0.05M Tris—HCl (pH7) .The crystal of TM was characterized with its needle-shape and revealed a typical and distinctive striations with a period 40nm.Some details could be seen on it.

关 键 词：鲟鱼 原肌球蛋白 电镜观察 

分 类 号：Q959.463[生物学—动物学]