PCR-SSCP检测肺癌EGFR基因突变  

Study on PCR-SSCP Detecting EGFR Mutations in Lung Cancer

作　　者：奉水东[1] 陈敏时[1] 陈新[1] 让蔚清[2] 凌宏艳[3] 

机构地区：[1]南华大学流行病学教研室,湖南衡阳421001 [2]南华大学卫生毒理学教研室,湖南衡阳421001 [3]南华大学生理学教研室,湖南衡阳421001

出　　处：《现代检验医学杂志》2008年第2期13-15,共3页Journal of Modern Laboratory Medicine

基　　金：湖南省卫生厅资助项目(B200695)

摘　　要：目的分析PCR-SSCP检测肺癌EGFR基因突变的敏感性。方法应用PCR-SSCP检测36例肺癌标本的突变情况并与其它文献进行比较。结果在36例肺癌标本中,有11例存在突变,突变率为30.6%(11/36),其中男性2例,女性9例。结论PCR-SSCP检测肺癌EGFR基因突变具有较高的敏感性,与其它文献报道一致。Objective To analyse sensitivity of PCR-SSCP on detecting EGFR gene mutations in lung cancers. Methods PCR-SSCP was employed to detect EGFR mutation in 36 samples with lung cancer. Then the mutation rates were calculated and compared with other relevant to documents. Results 11 of 36 lung cancer cases yielded the EGFR mutations,of which,2 were males and 9 were females. Conclusion It is sensitive for PCR-SSCP to detect EGFR mutation in lung can-cers.

关 键 词：聚合酶链反应 单链构象多态性 肺癌 表皮生长因子受体基因 检测 突变 

分 类 号：R734.2[医药卫生—肿瘤] Q754[医药卫生—临床医学]