脊髓p38MAPK在大鼠背根节慢性压迫神经病理性疼痛中的作用  

The role of p38MAPK of spinal cord in the neuropathic pain following chronic compression of dorsal root ganglion of rats

作　　者：胡建雷 申文[2] 曾因明[2] 

机构地区：[1]宁波市鄞州人民医院,浙江宁波315040 [2]徐州医学院江苏省麻醉医学研究所,江苏徐州221002

出　　处：《中国药理学通报》2008年第4期530-533,共4页Chinese Pharmacological Bulletin

基　　金：江苏省社会发展计划基金资助项目(NoBS2006016)

摘　　要：目的观察背根节慢性压迫(chronic compression of dorsal root ganglion,CCD)痛大鼠脊髓p-p38MAPK表达的变化,探讨p38MAPK与慢性神经病理性痛的相关性。方法♂SD大鼠36只,随机分为正常组(Naive组,n=4)、假手术组(Sham组,n=16)和背根节慢性压迫组(CCD组,n=16),Sham组和CCD组又分别分为5,7,14d和21d4个亚组(n=4),分别用免疫印迹法(Westernblot)检测各组大鼠脊髓磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)表达的变化。结果3组大鼠脊髓均见p-p38MAPK蛋白表达。Naive组和Sham组间比较差异无统计学意义。CCD组大鼠脊髓p-p38MAPK蛋白表达较Naive组和Sham组明显增多;与Sham组相比,CCD后5,7,14,21d各组大鼠脊髓背角神经元胞质p-p38MAPK蛋白分别增加了138·1%(P<0·01)、184·3%(P<0·01)、247·4%(P<0·01)和90·4%(P<0·05)。胞核p-p38MAPK蛋白分别增加了167·3%(P<0·01)、177·8%(P<0·01)、262·7%(P<0·01)和72·7%(P<0·05)。结论在CCD大鼠模型中,脊髓p38MAPK的活化与背根节慢性压迫致神经病理性痛的形成和发展存在密切联系。Aim To investigate the changes of phosphorylated p38 mitogen-activated protein kinase （p- p38MAPK） in the spinal cord following chronic compression of dorsal root ganglion （CCD）in rats, and to explore the correlation between chronic neuropathic pain and p38MAPK. Methods The animal model of neuropathic pain following chronic compression of dorsal root ganglion was established. 36 male Sprague- Dawley rats were randomly divided into Naive group（ n = g ）, Sham group （ n = 16 ）, CCD group （ n = 16 ）. Sham group and CCD group were also divided into 5, 7, 14 d and 21 d group respectively （ n = g ）, Western blot analysis was used to investigate the expression of the phosphorylated active form of p38MAPK （p- p38MAPK） in the spinal dorsal horn in rats after CCD. Results p-p38MAPK protein was expressed in spinal cord among all three groups, but there were no significant differences between Naive group and Sham group. Compared to Naive and Sham group, rats in CCD group showed significant increase of the expression of p-p38MAPK protein in spinal cord. Compared with that of Sham group, protein expression of p- p38MAPK in cytosol in the spinal dorsal horn in 5,7, 14 d and 21 d of post-surgery increased respectively 138.1%（P〈0.01）,184.3%（P〈0.01）, 247.4% （ P 〈 0. 01 ） and 90. 4% （ P 〈 0. 05 ） in CCD group ; and protein expression of p-p38MAPK in nuclear in the spinal dorsal horn in 5,7,14 d and 21 d of post-surgery increased respectively 167.3% （ P 〈 0. 01 ） , 177.8% （P〈0.01）,262.7% （P〈0.01）and 72.7% （P〈 0. 05 ）in CCD group. Conclusions Activation of p38MAPK contributes to the maintenance of neuropathic pain induced by chronic compression of dorsal root ganglion（CCD） in rats.

关 键 词：P38丝裂原活化蛋白激酶 背根节慢性压迫 神经病 理性痛 脊髓 

分 类 号：R-332[医药卫生] R322.81