双水相萃取法分离纯化白芸豆中α-淀粉酶抑制剂的研究  

Study on Purification of Alpha-Amylase Inhibitor by Aqueous Two-phase Extraction,from the Kidney Bean,Phaseolus Vulgaris

作　　者：张佰鹏[1] 高美风[1] 徐雯[1] 付金香[1] 孔毅[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2008年第2期129-132,共4页Pharmaceutical Biotechnology

摘　　要：采用PEG/(NH4)2SO4双水相系统萃取α-淀粉酶抑制剂,考察了PEG浓度、(NH4)2SO4浓度和NaCl溶液浓度对α-淀粉酶抑制剂分配系数、相比和活力回收率的影响。确定了白芸豆中α-淀粉酶抑制剂提取的最佳条件,即PEG质量分数为12.0%,(NH4)2SO4的质量分数为13.3%,NaCl质量分数为0.003%时,分配系数、相比和活力回收率分别为4.40,0.57,71.41%。Extraction of alpha-Amylase Inhibitor by of the Concentration of PEG, （NH4）2SO4 and NaCl Aqueous Two-phase Extraction was studied. Effect on extraction was investigated. The result indicated that the partition coefficient of 4.40 and the recovery of total activity of 71.41% could be obtained under the conditions of PEG2000（12.0%）, （NH4）2SO4 （13.3%）and NaCl（0. 003%）.

关 键 词：双水相萃取 Α-淀粉酶抑制剂 分离纯化 

分 类 号：R914[医药卫生—药物化学]