二氧化碳气腹对人卵巢癌细胞系SKOV_3裸鼠腹腔转移瘤生长的影响及机制探讨  

Effects of carbon dioxide pneumoperitoneum on the abdomino-metastatic tumor growth of human ovarian carcinoma cell line(SKOV_3) in nude mice and analysis of its mechanism

作　　者：唐均英[1] 黄金[1] 姚珍薇[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2008年第4期441-445,共5页Journal of Chongqing Medical University

摘　　要：目的:研究二氧化碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响,并初步探讨其机制。方法:构建裸鼠腹腔转移瘤的动物模型,模拟腹腔镜CO2气腹环境,通过观察肿瘤种植生长的情况,及TUNNEL法测定肿瘤组织凋亡情况,以探讨二氧化碳气腹对卵巢癌细胞裸鼠腹腔转移瘤生长的影响;通过免疫组织化学法和Western-blot测定肿瘤组织中VEGF及MMP-9表达,以探讨其可能存在的机制。结果:经CO2气腹处理后,腹腔内肿瘤质量大于对照组,肿瘤组织中VEGF和MMP-9蛋白的表达差异无显著性,各组间肿瘤细胞的凋亡率差异无显著性。结论:CO2气腹有促进卵巢肿瘤细胞SKOV3生长的作用。Objective：To investigate the effects of carbon dioxide（CO2） pneumoperitoneum on the abd growth of ovarian carcinoma cell in nude mice and to primarily analyze its mechanism. Methods：The nude mice model of abdomino-metastatic tumor of ovarian carcinoma cell was established, and laparoscopic CO2 pneumoperitoneum environment was simulated. Tumor implantation and growth were observed and tumor tissue apoptosis was detected by TUNNEL method,to explore the effect of CO2 pneumoperitoneum on the abdomino-metastatic tumor growth of ovarian carcinoma cell in nude mice. The expressions of VEGF and MMP9 in cancer tissue were determined by immunohistochemistry and Western Blot to analyze the possible mechanism. Results：After the treatment of CO2 pneumoperitoneum, the tumor quality in abdominal cavity was higher than that of the control group,the expression difference of VEGF and MMP9 in tumor tissues was not significant and tumor cell apoptosis rates of each group do not differ significantly. Conclusions ：Carbon dioxide pneumoperitoneum can promote the growth of ovarian carcinoma cell SKOV3.

关 键 词：CO2气腹 裸鼠 凋亡 VEGF MMP-9 

分 类 号：R737.31[医药卫生—肿瘤] R656[医药卫生—临床医学]