染料木苷和黄豆苷的纯化和分离  

Purification and Isolation of Soybean Genistin and Daidzin

作　　者：孙艳梅[1] 张永忠[1] 徐宝荣[1] 杨林 李木子[1] 迟玉杰[3] 

机构地区：[1]东北农业大学应用化学系,黑龙江哈尔滨150030 [2]哈尔滨三精制药有限公司,黑龙江哈尔滨150030 [3]教育部大豆生物学重点实验室

出　　处：《食品科学》2008年第4期189-192,共4页Food Science

基　　金：黑龙江省“十一五”重大科技攻关项目(GA06B402-4);大豆生物学教育部重点实验室主任基金项目(SB05A04)

摘　　要：为了深入研究大豆提取物中主要成分染料木苷和黄豆苷单体的性质,本实验进行了二者的分离。首先用丙酮萃取和无水乙醇重结晶等方法纯化大豆异黄酮提取物,得到纯度较高的染料木苷和黄豆苷混合物。然后,利用Sephadex LH-20柱层析实现了染料木苷和黄豆苷的分离。In order to study the properties of respetive monomer of genistin and daidzin as main compounds of isoflavone extracts from soybean, their respective isolation was studied in the paper. First, in order to acquire high Purity of products, study purified isoflavone extracts by acetone extraction with anhydrous alcohol recrystaUization. Then, genistin and daidzin were respectively separated by Sephadex LH-20 column.

关 键 词：大豆异黄酮 染料木苷 黄豆苷 纯化 分离 SEPHADEX LH-20 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS214.2[轻工技术与工程—食品科学与工程]