韭菜中农药残留酶速测法假阳性消除研究  

Study on Elimimating False Positive Results of Pesticide Residues in Leek with Enzyme Screening Method

作　　者：葛静[1] 王素利[1] 钱传范[1] 江树人[1] 赵婴荣 张敬锁[3] 刘丰茂[1] 

机构地区：[1]中国农业大学理学院,北京100094 [2]北京市农业环境监测站,北京100029 [3]河北北方学院,河北张家口075131

出　　处：《食品科学》2008年第4期299-301,共3页Food Science

摘　　要：酶速测方法是基于不同有机磷农药和氨基甲酸酯农药对胆碱酯酶活性抑制程度不同而对农药残留快速检测的方法。但韭菜等样本经常出现检测假阳性问题。本实验针对韭菜在农药残留酶速测中出现的假阳性问题,对样本前处理方法进行改进,以消除实验中出现的假阳性。研究了韭菜样本剪切长度以及在加酶前对样本提取液进行加热处理后的效果。实验结果表明,该方法在一定范围内可以消除韭菜的假阳性问题。Enzyme screening method was simple and rapid to determine the pesticide residues based on the butyryl cholinesterase activity inhibition by both organic phosphorous and carbamate pesticide residue in the sample. Nevertheless false positive results in leek samples were reported because of the assay interferences with leek. In this study the heat treatment was applied to eliminate the false positive results of pesticide residue in leek, so the sample preparation procedure was improved and the results showed that the test sample heating treatment can temperature and time decrease the false positive result.

关 键 词：酶速测 假阳性 韭菜 

分 类 号：TS207.53[轻工技术与工程—食品科学]