番茄红素对去势大鼠骨量和骨生物力学的影响  

Effect of lycopene on bone mass and biomechanics in ovariectomized rats

作　　者：杨希重[1] 季翠杰 陈晓亮[1] 李磊[1] 

机构地区：[1]青岛大学医学院附属医院脊柱外科,山东省青岛市266003 [2]蓬莱市第二人民医院内科,山东省蓬莱市265612

出　　处：《中国组织工程研究与临床康复》2008年第15期2811-2814,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：民政部十一五课题项目,民人教科学[(2007)18-1-27]~~

摘　　要：目的:番茄红素作为自然界中的抗氧化剂,能够减低人类某些与年龄老化有关的疾病的发生。实验拟进一步验证番茄红素对实验性骨质疏松大鼠骨密度和骨生物力学的影响。方法:实验于2006-07/2007-11在青岛大学医学院附属医院动物实验基地完成。番茄红素油树脂由新疆智星科技投资开发有限公司提供,使用时玉米油稀释至所需浓度。选择6个月龄SPF级雌性wistar大鼠50只,按体质量随机分成5组,即假手术+玉米油组(2mL/d)、卵巢切除+玉米油组(2mL/d)、卵巢切除+苯甲酸雌二醇组(0.2mg/kg,1次/周)、番茄红素低剂量组(10mg/kg,1次/d)、番茄红素高剂量组(20mg/kg,1次/d)。每组10只,除假手术组外其余各组均切除双侧卵巢,建立骨质疏松动物模型,按组给予灌服给药,治疗12周。结果:术后2周内假手术组死亡1只,低剂量组死亡2只。47只大鼠进入统计学分析。①卵巢切除组大鼠腰椎及股骨的骨密度和最大应力均较假手术组降低,差异有显著性(P<0.05)。②摄取番茄红素和雌激素的大鼠,股骨及腰椎骨密度和最大应力比卵巢切除组明显升高,差异有显著性(P<0.05)。③番茄红素增加骨密度和提高最大应力作用不如雌激素明显;高剂量组作用强于低计量组。结论:番茄红素可提高骨质疏松大鼠的骨密度和骨生物力学特性,但其作用低于雌激素,是否与用药时间和样本例数不多有关尚需观察。AIM： Lycopene as an antioxidant can decrease the risk of age-related chronic diseases, such as cancer. In this study, we investigated the impact of lycopene on bone mineral density and bone biomechanics in experimental osteoporotic rats. METHODS： The experiment was performed at the Animal Experiment Center of the Affiliated Hospital of Qingdao University Medical College from July 2006 to November 2007. The lycopene was provided by the Xinjiang Zhixing Technology Invest Development Company, and diluted to certain concentration by corn oil. Fifty six-month-old SPF female Wistar rats were selected and divided into 5 groups （n=10） according to body mass： Sham operation and corn oil group （2 mL/d）, ovariectomy （OVX） and corn oil group （2 mL/d）, OVX and estradiol benzoate （EB） group （0.2 mg/kg, once a week）, low and high lycopene groups （10 mg/kg, 20 mg/kg, once a day）. Except the sham operation group, all rats underwent OVX to establish models of osteoporosis. Each group was administrated corresponding medicine for 12 weeks. RESULTS： Within 2 weeks postoperatively, 1 of sham operation group died and 2 of low lycopene group died. Finally, 47 rats were included in final analysis. （1）The lumbar and femoral bone mineral density and maximum stress of ovariectomized rats were significantly lower than those of sham operation group （P 〈 0.05）. （2）The lumbar and femoral bone mineral density and maximum stress of rats treated by lycopene and EB were significantly greater than those in ovariectomized rats （P 〈 0.05）. （3）Lycopene could increase bone mineral density and maximum stress, but its effect was inferior to EB. In addition, high dose group had stronger effect compared with low dose group. CONCLUSION： Lycopene can improve the bone mineral density and biomechanics of ovariectomized rats, but the effect is lower than EB, which may be correlated with administration time and sample quantity.

关 键 词：番茄红素 骨质蔬松 骨密度 生物力学 组织构建 

分 类 号：R681.4[医药卫生—骨科学]