RP—HPLC快速分析牛奶中六种磺胺类抗生素残留  

Analysis of six sulfonamide residues in milk with a rapid RP-HPLC method

作　　者：孙晶玮[1] 赵新淮[1] 

机构地区：[1]乳品科学教育部重点实验室东北农业大学,黑龙江哈尔滨150030

出　　处：《食品工业科技》2008年第4期274-276,278,共4页Science and Technology of Food Industry

基　　金："十一五"国家奶业专项

摘　　要：研究了牛奶中磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲基嘧啶、磺胺甲噁唑、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶六种磺胺类抗生素的多残留反相高效液相色谱(RP-HPLC)分析方法。样品经超声波提取两次,提取液浓缩至干。残渣用流动相甲醇-0.08%乙酸(60:40,V/V)溶解,净化后以反相C18柱,甲醇-0.08%乙酸等度洗脱,紫外检测器检测、外标法定量。结果表明,这六种抗生素在0.01～1.0mg.L-1浓度范围内呈线性关系,磺胺嘧啶、磺胺甲基嘧啶、磺胺二甲基嘧啶的检出限为0.010mg.L-1、磺胺甲噁唑、磺胺间甲氧嘧啶、磺胺间二甲氧嘧啶的检出限为0.015mg.L-1。分析的添加回收率达到80%以上,变异系数<9.0%,整体分析时间小于3h。方法具有处理简单、检测快速、准确的特点。The analysis method of six sulfonamides in milk, including sulfamerazine, sulfamethazine, sulfamethoxazole, sulfamonomethoxine, sulfadimethoxine, sulfadiazine was studied. Milk containing sulfonamides was extracted by ultrasonic method twice. The extract was combined and evaporated to dry, then dissolved in methanol-0.08% acetic acid （60：40, V/V）. Purified by hexane, the extract was analyzed in HPLC in a C18 column with methanol-acetic acid-H2O as eluent. Sulfonamide residues were detected by a UV detector and their concentrations were determined with calibrated curves. A good linear relationship existed for both residues in concentration range of 0.01-1.0mg · L^-1, and detection limit for sulfadiazine and sulfamerazine and sulfamethazine was 0.010mg · L^-1 and for sulfadimethoxine and sulfamonomethoxine and sulfadimethoxine was 0.015mg · L^-1. The analysis procedure needs total time less than 3h with an addition recovery for sulfonamides about 80% and coefficient of variation less than 9.0%. The method was rapidly, simply and accurately.

关 键 词：磺胺 牛奶 分析 反相高效液相色谱 

分 类 号：TS207.5[轻工技术与工程—食品科学]