Effects of α-adrenoreceptor antagonists on apoptosis and proliferation of pancreatic cancer cells in vitro  

作　　者：Su-Gang Shen Dong Zhang Heng-Tong Hu Jun-Hui Li Zheng Wang Qing-Yong Ma 

机构地区：[1]Department of Hepatobiliary and Pancreas Surgery, The First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China

出　　处：《World Journal of Gastroenterology》2008年第15期2358-2363,共6页世界胃肠病学杂志（英文版）

基　　金：The Natural Science Foundation of Shaanxi Province, No. 2006C209

摘　　要：AIM: To discuss the expression of α-adrenoreceptors in pancreatic cancer cell lines PC-2 and PC-3 and the effects of α1- and α2-adrenoreceptor antagonists, yohimbine and urapidil hydrochloride, on the cell lines in vitro. METHODS: We cultured the human ductal pancreatic adenocarcinoma cell lines PC-2 and PC-3 and analyzed the mRNA expression of α1- and α2-adrenergic receptors by reverse transcription polymerase chain reaction (RT-PCR). The effects of yohimbine and urapidil hydrochloride on cell proliferation were assessed by 3-(4,5-dimethylthiasol-2-yl)- 2,4,-diphenyltetrazolium bromide (MTT) assay. Apoptosis was detected using the terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) assay and flow cytometry (FCM). RESULTS: PC-2 expressed mRNA in α1- and α2- adrenoreceptors. MTT assays showed that urapidil hydrochloride had no effect on PC-3 cell lines. However, exposure to urapidil hydrochloride increased DNA synthesis in PC-2 cell lines as compared to the control group. PC-2 cell lines were sensitive to both drugs. The proliferation of the 2 cell lines was inhibited by yohimbine. Cell proliferation was inhibited by yohimbine via apoptosis induction. CONCLUSION: The expression of α1- and α2- adrenoreceptors is different in PC-2 and PC-3 cell lines, which might be indicative of their different functions. The α2-adrenoceptor antagonist, yohimbine, can inhibit theproliferation of both cell lines and induce their apoptosis, suggesting that yohimbine can be used as an anticancer drug for apoptosis of PC-2 and PC-3 cells.AIM： To discuss the expression of α-adrenoreceptors in pancreatic cancer cell lines PC-2 and PC-3 and the effects of α1- and α2-adrenoreceptor antagonists, yohimbine and urapidil hydrochloride, on the cell lines in vitro. METHODS： We cultured the human ductal pancreatic adenocarcinoma cell lines PC-2 and PC-3 and analyzed the mRNA expression of α1- and α2-adrenergic receptors by reverse transcription polymerase chain reaction （RT-PCR）. The effects of yohimbine and urapidil hydrochloride on cell proliferation were assessed by 3-（4,5-dimethylthiasol-2-yl）- 2,4,-diphenyltetrazolium bromide （MTT） assay. Apoptosis was detected using the terminal deoxyribonucleotidyl transferase （TdT）-mediated biotin-16-dUTP nick-end labeling （TUNEL） assay and flow cytometry （FCM）. RESULTS： PC-2 expressed mRNA in α1- and α2- adrenoreceptors. MTT assays showed that urapidil hydrochloride had no effect on PC-3 cell lines. However, exposure to urapidil hydrochloride increased DNA synthesis in PC-2 cell lines as compared to the control group. PC-2 cell lines were sensitive to both drugs. The proliferation of the 2 cell lines was inhibited by yohimbine. Cell proliferation was inhibited by yohimbine via apoptosis induction. CONCLUSION： The expression of α1- and α2- adrenoreceptors is different in PC-2 and PC-3 cell lines, which might be indicative of their different functions. The α2-adrenoceptor antagonist, yohimbine, can inhibit theproliferation of both cell lines and induce their apoptosis, suggesting that yohimbine can be used as an anticancer drug for apoptosis of PC-2 and PC-3 cells.

关 键 词：Alpha-adrenoreceptor Pancreatic cancer YOHIMBINE Urapidil hydrochloride 

分 类 号：R735.9[医药卫生—肿瘤]