STAT3反义寡核苷酸联合放疗对喉癌Hep-2细胞周期的影响  

Effect of STAT3 antisense oligonucleotide and radiotherapy on hep-2 cells cycle

作　　者：海占兵[1] 李晓明[2] 路秀英[2] 

机构地区：[1]中国人民解放军第三军医大学,重庆400038 [2]解放军白求恩国际和平医院

出　　处：《山东医药》2008年第12期37-39,共3页Shandong Medical Journal

基　　金：全军医药卫生十一五科研资助项目(200606MA076)

摘　　要：目的探讨STAT3反义寡核苷酸(STAT3 ASODN)联合放疗对人喉癌Hep-2细胞增殖及周期的影响。方法体外培养Hep-2细胞,被STAT3 AS-ON转染后,将所有细胞分为放疗组与未放疗组,放疗组根据转染复合物的不同分为反义+放疗组、正义+放疗组及单纯放疗组(放疗对照组),未放疗组分为反义组、正义组及空白对照组;各组于37℃温箱内继续培养,放疗组Hep-2细胞于培养24 h后给予5 Gy60Coγ射线照射后,与未放疗组再继续培养24 h,流式细胞术检测细胞周期情况。结果反义+放疗组作用于Hep-2细胞后G0/G1期细胞比例(73.13±3.94)较正义+放疗组(55.39±2.69)及单纯放疗组(54.61±3.69)明显提高(P<0.01),S期细胞比例(22.09±2.83)较正义+放疗组(30.60±2.27)及单纯放疗组(31.31±1.89)明显下降(P<0.01);反义+放疗组增殖指数(PI值)(0.269±0.394)与正义+放疗组(0.446±0.269)及单纯放疗组(0.454±0.327)相比明显下降(P<0.01)。结论STAT3 AS-ON联合γ射线作用于Hep-2细胞后,可以抑制G1-S期的转化,同时,细胞增殖抑制作用明显增强。Objective To study the effect of STAT3 antisense oligonucleotide （STAT3 ASODN） in combination with radiotherapy on proliferation and Hep-2 cells cycle cells in human laryngeal cancer. Methods Hep-2 cells were cultured in vitro after Hep-2 cells was transfected with STAT3 ASODN, and all the cells were divided into two-groups about the radiotherapy group and the non-radiotherapy group. According to the difference of the transfection complex, radiotherapy group was divided into antisense ＋ radiotherapy group, sense ＋ radiotherapy group, and simple radiotherapy group （ radiotherapy control group） similarly, the non-radiotherapy group were divided into antisense group, sense group, and blank control group, and then they were cultured continuously in the incubator at 37 ℃ and the Hep-2 cells of the radiotherapy group were irradiated by γ-rays （5 Gy） after 24 h. Flow cytometry was performed to analyze all the cells cycle when the cells of the radiotherapy group were irradiated after 24h. Results The percentage of G0/G1 phase cell in antisense ＋ radiotherapy group （73.13±3.94） was higher than the sense ＋ radiotherapy （55.39±2. 69 ） and simple radioterapy groups （54. 61±3.69 ） （ P 〈 0. 01 ） ; and the percentage of S phase cell （ 22.09±2. 83 ） in antisense ＋ radiotherapy group （ 73.13±3.94 ） was lower than the sense ＋ radiotherapy （ 30. 60±2.27 ） and simple radioterapy groups （ 31.31±1.89） （ P 〈 0. 01 ） ; the proliferation index （PI） responded the proliferative ability of cells in antisense ＋ radiotherapy group （0. 269±0. 394） was lower than the sense ＋ radiotherapy （0. 446±0. 269） and simple radioterapy groups （0. 454±0. 327 ） （ P 〈 0. 01 ）. Conclusion STAT3 AS-ON and γ-rays can suppress the transformation of G1 -S phase and the growth if they act on Hep-2 cells at one time.

关 键 词：喉肿瘤 反义寡核苷酸 放射疗法 细胞周期 

分 类 号：R739.6[医药卫生—肿瘤]