Slit2和Robo1蛋白表达与胃癌关系的临床研究  

Slit2/Robo1 signaling expression and its correlation with angiogenesis in gastric cancers

作　　者：季锐[1] 卢雪峰[1] 付金栋[1] 姜旭生[2] 雒燕[1] 杨淑萍[1] 

机构地区：[1]山东大学齐鲁医院消化内科,山东济南250012 [2]山东大学齐鲁医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》2008年第2期117-120,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究胃癌组织中神经迁移因子Slit2与受体Robo1的表达,探讨两者与胃癌生物学行为和血管生成的关系。方法:免疫组织化学SP法检测54例胃癌组织及28例癌旁组织中Slit2蛋白和Robo1蛋白的表达水平,观察不同临床病理学行为下Slit2蛋白和Robo1蛋白的表达变化;检测CD34标记的微血管密度(microvessel density,MVD),研究在胃癌组织中MVD与Slit2蛋白和Robo1蛋白的关系。结果:Slit2蛋白和Robo1蛋白在胃癌组织中的阳性表达率分别为63.0%和77.8%,在癌旁组织中的阳性表达率分别为3.6%和10.7%,两种组织中Slit2蛋白和Robo1蛋白的表达水平差异有统计学意义(χ2=26.586,P<0.01;χ2=33.491,P<0.01)。胃癌组织中MVD显著增高(t=19.562,P<0.01)。Slit2蛋白表达水平与肿瘤大小、TNM分期、淋巴结转移和远处转移相关;Robo1蛋白表达水平与肿瘤大小、浸润深度、TNM分期、淋巴结转移相关。Slit2与Robo1阳性表达呈正相关(r=0.4202,P<0.01)。Slit2和Robo1表达阳性者MVD显著增高(t=2.256,P<0.05;t=2.631,P<0.05)。结论:Slit2及其受体Robo1在胃癌组织中表达增加,与肿瘤的侵袭和转移密切相关,其机制可能是Slit2/Robo1信号途径能促进肿瘤血管生成。Objective To investigate the expressions of Slit2 and its receptor Robol in human gastric carcinomas. Methods The expression of Slit2 protein, Robol protein and CD34-1abeled microvessel density （MVD） were measured by immunohistochemical staining （SP） in 54 cases of gastric carcinomas and 28 cases of Para-cancer tissues, Results：The positive rates of Slit2 ,Robol were 63.0% and 77.8% and the expression of Slit2, Robol and MVD in cancerous tissues were higher than those in para-cancer tissues2 （X^2=26.586, P 〈 0.01,X^2=33.491, P 〈 0.01, t= 19.562, P 〈 0.01）. The expression rates of Slit2 and Robol were significantly associated with tumor size,TNM stage, invasion depth and lymph node metastasis. Robol was also associated with distant metastasis. MVD in Slit2/Robo1-positive tissues was much higher than that in negative tissues （t=2.256, P〈0.05,t=2.631, P〈0.05）,there was a significant positive correlation between Slit2 and Robol expression （r=0.4202, P〈0.01）. Conclusion Slit2/Robo1 signaling were distinctively overexpressed and might play a key role in the invasion and metastasis of gastric cancer.

关 键 词：胃肿瘤 SLIT ROBO 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]