亚硝酸钠对大鼠睾丸支持细胞DNA损伤的研究  

STUDY ON DNA DAMAGE INDUCED BY SODIUM NITRITE IN THE SERTOLI CELLS OF RATS

作　　者：吴赤蓬[1] 张晓蓉[1] 韩辉[2] 宿宝贵[2] 

机构地区：[1]暨南大学医学院公共卫生学教研室,广州510632 [2]暨南大学医学院解剖学教研室

出　　处：《现代预防医学》2008年第9期1688-1690,共3页Modern Preventive Medicine

基　　金：国家211工程项目;广东省科技计划项目(2003C20419)

摘　　要：[目的]观察亚硝酸钠对大鼠睾丸支持细胞DNA的损伤作用。[方法]采用冷胰酶消化法分离培养大鼠睾丸支持细胞,采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞DNA的损伤作用。试验共设6个剂量组,亚硝酸钠终浓度分别为1.5μg/ml、15μg/ml、150μg/ml和1500μg/ml,同时设PBS阴性对照组和丝裂霉素C阳性对照组(20μg/ml)。[结果]当亚硝酸钠剂量为15μg/ml时,可引起支持细胞拖尾率的增加,与阴性对照组相比,其差异有统计学意义(χ2=18.320,P=0.000),但其尾长、尾/头(长)、尾距等较为客观的距离指标与阴性对照组相比,则在亚硝酸钠剂量达150μg/ml时,差异才有统计学意义,随着染毒剂量的增加,DNA损伤程度明显加重,存在明显的剂量-效应关系。[结论]亚硝酸钠具有损伤睾丸支持细胞DNA的作用,并且在染毒剂量为150μg/ml时,能观察到明显的损伤效应。[Objective] To detect the DNA damage induced by sodium nitrite in the sertoli cells of rats. [Methods] The sertoli cells were isolated with the cold trypsin digestion method. Single-cell gel electrophoresis （SCGE） was used to detect the DNA damage induced by sodium nitrite in the sertoli cells. There were six dose groups in this test. They included 1.5μg/ml, 15μg/ml, 150 μg/ml and 1500μg/ml concentration groups of sodium nitrite, PBS negative conLrol group and mitomyein C （20μg/ml） positive conLrol group. [Results] When the dose of sodium nitrite was 15μg/ml, the rate of tailing cells had a signif- icant difference comparing with the negative control group （x^2= 18.320, P=0.000）. But in the objective variables such as tail length, the tail/head （length）, tail moment, this group had no significant difference comparing to negative control group. In the dose of 150μg/ml, the difference in all variables was significant. As the dose increased, the extent of DNA damage increased significantly. There was an obvious dose-effect relationship. [Conclusion] NaNO2 can induce DNA damage in sertoli cells of rates, but only when the dose at 150μg/ml. the effects of injury can be observed clearly.

关 键 词：亚硝酸钠 睾丸支持细抱 彗星试验 单细胞凝胶电泳 DNA损伤 

分 类 号：R394[医药卫生—医学遗传学]