腺病毒介导的人CD40配体基因抑制裸鼠卵巢癌生长的实验研究  

EXPERIMENTAL STUDY OF ADENOVIRUSMEDIATED HUMAN CD40 LIGAND GENE IN INHIBITING THE GROWTH OF NUDE MICE'S OVARIAN CANCER

作　　者：吴红兵[1] 田聆[2] 王虎[1] 文艳君[2] 魏于全[2] 郑广宁[1] 

机构地区：[1]四川大学华西口腔医院放射科,四川成都610041 [2]四川大学华西医院生物治疗国家重点实验室,四川成都610041

出　　处：《现代预防医学》2008年第9期1736-1737,1740,共3页Modern Preventive Medicine

基　　金：国家自然基金资助项目(10675089)

摘　　要：[目的]探讨重组腺病毒人CD40配体基因(Ad-hCD40L)对卵巢癌的治疗作用。[方法]建立卵巢癌裸鼠动物模型,检测腺病毒介导的人CD40配体基因对肿瘤生长的抑制作用。[结果]瘤内注射Ad-hCD40L明显抑制裸鼠卵巢癌生长,Tunel法检测和HE染色肿瘤组织石腊切片观察调亡细胞明显增多,并可见大量肿瘤细胞坏死。[结论]腺病毒介导的人CD40配体基因抑制裸鼠肿瘤生长,增强诱导肿瘤细胞调亡,为卵巢癌的基因治疗提供了新的途径。[Objective] To investigate the inhibitory, effect in treating ovarian cancer with recombinant adenovirusmediated human CD40 ligand gene （Ad-hCD40L）. [Methods] A nude mice＇s ovarian cancer model was built to exam the andcancer effect of recombinant Ad-hCD40 on the tumor growth. [ Results] Human CD40 ligand gene could suppress the growth of tumor in nude mice by intratumoral injection with Ad-hCD40L. Immunohistochemical and HE staining revealed that in Ad-hCD40L treatment group apoptosis obviously increased and a great of tumor cells occurred necrosis comparing with other groups. [Conclusion] The recombinant Ad-hCD40L could suppress the growth of tumor and induce more apoptosis and necrosis in ovarian cancer ceils. These findings may provide a new strategy in treating ovarian cancer with gene therapy.

关 键 词：人CD40配体 重组腺病毒载钵 裸鼠卵巢癌 基因治疗 

分 类 号：R737.31[医药卫生—肿瘤]