“O”型口蹄疫病毒生物合成多肽苗保护性抗体的免疫应答  

IMMUNOLOGIC RESPONSE OF PROTECTIVE ANTIBODY AGAINST SEROTYPE“O”FMDV USING BIOSYNTHETIC PEPTIDE VACCINE

作　　者：尤永进[1] 徐泉兴[1] 赵洪兴[1] 饶忠[1] 乌正芳 郑兆鑫[2] 郭杰炎[2] 金建平[2] 盛祖恬[3] 何秉耀[3] 何永强[3] 杜青云[3] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]复旦大学,上海200433 [3]浙江省农业科学院病毒研究所,杭州310021

出　　处：《上海农业学报》1997年第4期1-3,共3页Acta Agriculturae Shanghai

摘　　要：pXZ500质粒表达的生物合成多肽苗（20肽-14肽-20肽）分别以总剂量32μg／头和0．346mg／头分两次免疫接种豚鼠和猪，均可获得保护性抗体的良好免疫应答。豚鼠和猪在第一次免疫接种后40d和45d时，其血清的乳鼠保护指数均在2．0以上；分别用50个发病单位强毒攻击，均可获得100％保护。The good immunologic responses of protective antibody have been producedin guinea pigs and swine, which were vaccinated using biosynthetic peptide (20AA-14AA-20AA)vaccine expressed in pXZ500. Guinea pig inoculated on days 0 and 21 with 16 μg dosesof 20AA-14AA-20AA was fully protected on day 40 against challenge of virulent FMDV.The protective indexes of antibody against FMDV in sucking mouse protection test are over2. 0. It has also been shown that the same results as in guinea pig test concerning protectionand protective index have been obtained in swine inoculated on days 0 and 21 with 0. 346mgdoses of the same vaccine.

关 键 词：FMDV 生物合成 多肽苗 免疫应答 口蹄疫病毒 

分 类 号：S859.797[农业科学—临床兽医学] S855.3[农业科学—兽医学]