30例配对新鲜胃癌标本中p53基因突变的研究  

The Research on p53 Gene Mutations in 30 Paired Cases of Fresh Gastric Carcinoma Biopsies

作　　者：刘腾飞[1] 尹志华[2] 甘润良[3] 赵强[4] 董琳[3] 周建国[3] 冬毕华[3] 

机构地区：[1]南方医科大学肿瘤研究所,广州510515 [2]深圳市第四人民医院,深圳518040 [3]南华大学肿瘤研究所,衡阳421001 [4]南华大学附属第一医院病理科,衡阳421001

出　　处：《肿瘤预防与治疗》2008年第2期130-132,F0003,共4页Journal of Cancer Control And Treatment

基　　金：湖南省教委科研基金资助

摘　　要：目的：探讨胃癌标本中p53基因外显子5～8的突变情况及其与胃癌各临床特征的关系。方法：采用银染PCR—SSCP检测30例配对新鲜胃癌手术标本中p53基因外显子5～8的突变。结果：p53基因突变检出率为46．7％（14／30），p53基因外显子5—8的突变率分别为10％、16．7％、23．3％、16．7％；p53基因突变与病人年龄、性别、原发灶部位及肿瘤分化程度之间差异没有显著性意义（P〉O．05）；但是，p53基因突变与胃癌临床分期、淋巴结转移之间差异有显著性意义（P〈O．05），进展期胃癌p53基因突变高于早期胃癌，有淋巴结转移的胃癌组织中p53基因突变高于无淋巴结转移胃癌组织。结论：p53基因突变可能参与了早期胃癌一进展期胃癌的发生发展过程，并可能是胃癌发生淋巴结转移的机制之一。Objective： To investigate the mutations of p53 exon 5 - 8 in gastric carcinoma biopsies and their relationship with gastric clinicopathologic features. Methods： Silver staining PCR - SSCP method was used to detect the mutations of p53 exon 5 - 8. Results： The mutation detecting rate of p53 gene was 46.7% （14/30） in the gastric carcinoma samples and the p53 mutation detecting rates in exon 5-8 were 10%, 16.7%, 23.3% and 16.7% respectively. There were no significant correlation between p53 mutation detecting rate and the patient＇s age, sex, the differentiation degree of tumor cells, and the primary lesion＇s position （ P 〉 0.05 respectively）, while the p53 mutation detecting rate was correlated with the clinical classification （ early or advanced） and lymph node metastases （P 〈 0.05 respectively）. Conclusion： Our study suggests： 1. p53 mutation may contribute to the development of gastric carcinomas from early stages to advanced stages; 2. p53 mutation may be one of the mechanisms involved in the lymph node metastases of gastric carcinoma.

关 键 词：胃癌 P53基因 突变 

分 类 号：R735.2[医药卫生—肿瘤]