洗涤剂用碱性纤维素酶的研究(I)——产酶菌种的选育及发酵条件  

Alkaline Cellulase for Laundry Detergent(I)——Strain Breeding and Fermentation conditions

作　　者：余晓斌[1] 全文海[1] 章克昌[1] 

机构地区：[1]无锡轻工大学生物工程学院

出　　处：《工业微生物》1997年第4期5-8,共4页Industrial Microbiology

基　　金：国家"八五"科技攻关项目

摘　　要：从土壤中分离获得碱性纤维素酶产生菌嗜碱芽孢杆菌ＳＨＹ８－１，经紫外线（ＵＶ）、亚硝基胍（ＮＴＧ）、甲基磺酸乙酯（ＥＭＳ）诱变及反复的自然分离和压力选择，获得高产变异株ＳＨＹ８－５７２５，产酶量由０．８９８ｕ／ｍｌ提高到１５～２０ｕ／ｍｌ，对影响产酶的各因素进行了分析测试。Alkalophilic Bacillus SHY81 producing alkaline cellulase was isolated from soil. The strain was mutagenized by UV, NTG, EMS and was selected by pure culture and with pressure separation repeatedly. Then a hyperproductive mutant SHY85725 was obtained. The enzyme yield was increased from 0.898u/ml to 1520u/ml. The effects of various factors on enzyme production were examined.

关 键 词：碱性纤维素酶 嗜碱芽隐杆菌 诱变育种 发酵 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] TQ920.1