Anti-miR-155反义寡核苷酸对人肺鳞癌H157细胞增殖的影响  

Effect of anti-miR-155 oligonucleotides on proliferation of human lung squamous cell carcinoma cell line H157

作　　者：曹学武[1] 安江洪[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤研究所,重庆400042

出　　处：《西南国防医药》2008年第3期322-324,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金项目(30672076);国家重点基础研究发展"863"计划资助(2007AA02Z129)

摘　　要：目的:观察anti-miR-155反义寡核苷酸(AMOs)对肺鳞癌H157细胞增殖的影响。方法:将H157细胞分为对照组和AMOs处理组,AMOs处理组采用AMOs抑制H157细胞内miR-155的活性,液闪计数仪测定[3H]-TdR掺入量,MTT法测定细胞增殖抑制率,流式细胞仪测定细胞周期。结果:与对照组相比,AMOs显著减少H157细胞[3H]-TdR掺入量(P<0.01),随着浓度从10 nmol/L逐渐增加至于100 nmol/L,H157细胞[3H]-TdR掺入量亦随之减少(P<0.01);AMOs显著抑制H157细胞的增殖(P<0.01),使G0/G1细胞比例显著增加,G2/M期细胞比例显著减少(P<0.01)。结论:AMOs通过抑制H157细胞内高水平表达miR-155的活性,显著抑制H157细胞的增殖。Objective： To determine the effect of anti - miR - 155 oligonucleotides （AMOs） on proliferation of human lung squamous cell carcinoma cell line H157. Methods：Cultured H157 cells were divided into two groups： control group and AMOs group, In AMOs group, AMOs were used to inhibit the activity of miR - 155. [^3H]-TdR incorporation was detected by liquid scintillation counting, MTT assay was used to examine the inhibition percentage of AMOs to the proliferation of H157 cells and cell cycle was measured by flow cytometry. Results： AMOs decreased significantly the [^3H]-TdR incorporation in a dose - dependent manner （P〈0. 01）. With the increment of concentration of AMOs, the inhibition percentage increased significantly （P〈0. 01）. AMOs decreased significantly the cell ratios of G2/M phase and S phase, simultaneously increased the cell ratio of G0/G1 phase （P〈0.01）. Conclusion： AMOs inhibit the activity of miR - 155 expressing highly in H157 cells; as a result, inhibit significantly the proliferation of H157 cells.

关 键 词：H157 MIR-155 反义寡核苷酸 细胞增殖 

分 类 号：R730[医药卫生—肿瘤]