Genistein对腺样囊性癌细胞SACC-83中细胞凋亡相关蛋白表达的影响  

Effects of genistein on the expressions of cell apoptosis-related proteins in salivary adenoid cystic carcinoma cell line SACC-83

作　　者：马杰[1] 王洁[2] 钟鸣[2] 王兆元[2] 

机构地区：[1]中国医科大学附属盛京医院口腔科,辽宁沈阳110004 [2]中国医科大学口腔医学院病理科,辽宁沈阳110002

出　　处：《上海口腔医学》2008年第2期196-199,共4页Shanghai Journal of Stomatology

基　　金：基金]辽宁省教育厅科学研究计划资助项目(05L533)

摘　　要：目的:研究酪氨酸蛋白激酶抑制剂Genistein诱导人唾液腺腺样囊性癌细胞株SACC-83细胞凋亡的分子机制。方法:用Genistein处理体外培养的SACC-83细胞,采用Western印迹技术检测bax、bcl-2和survivin蛋白的表达,并利用电泳凝胶成像分析软件,对其结果进行量化分析,采用SPSS11.5软件包对结果进行方差分析。结果:随着Genistein作用时间的延长和浓度的增加,bax蛋白的表达明显增加,bcl-2和survivin蛋白的表达明显减少。SACC-83细胞经220μmol/LGenistein作用3d,其bax蛋白的表达量是对照组的3.43倍(P<0.01),而bcl-2和survivin蛋白的表达量分别是对照组的85%(P<0.05)和35%(P<0.01)。结论:Genistein诱导SACC-83细胞凋亡,与其上调bax蛋白的表达,以及下调bcl-2和survivin蛋白的表达有关。PURPOSE： To investigate the molecular mechanism of cell apoptosis induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83. METHODS： SACC-83 cells cultured in vitro were treated with genistein, the expressions of bax, bcl-2 and survivin proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software package. RESULTS： With the increase of concentration of genistein and the elongation of time, the expression of bax protein was significantly increased, but the expression of bcl-2 and survivin proteins was significantly decreased. Treated with 220μmol/L genistein for 3 days, the expression level of bax protein was 3.43 folds of the control group （P〈0.01）,but the expression level of bcl-2 and survivin proteins was respectively 85%（P〈 0.05） and 35%（P〈0.01 ） of the control group. CONCLUSION： The cell apoptosis induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the upregulation of bax protein expression,and the downregulations of bcl-2 and survivin proteins expression. Supported by Scientific Research Plan of Education Bureau of Liaoning Province（Grant No.05L533）.

关 键 词：酪氨酸蛋白激酶 GENISTEIN 唾液腺 腺样囊性癌 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]