apc基因突变与B细胞性非霍奇金淋巴瘤发病机理相关性的研究  

Study on the Relationship of the apc Gene Mutation and the Pathogenesis of B-cell Non-Hodgkin Lymphoma

作　　者：陈芳[1] 阮培刚[1] 邓飞[2] 

机构地区：[1]遵义医学院附属肿瘤医院,563003 [2]遵义医学院附属医院病理科,563003

出　　处：《实用癌症杂志》2008年第3期224-226,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金资助项目(编号:30160030)

摘　　要：目的探讨apc基因第15外显子密集突变区(Mutation cluster region,MCR)突变在B细胞性非霍奇金淋巴瘤(B-NHL)发生、发展中的作用。方法应用石蜡组织DNA抽提、PCR扩增和2%琼脂糖凝胶电泳技术检测40例B-NHL和20例直肠腺癌(阳性对照)组织中DNA的apc基因MCR突变情况。结果在相同PCR反应体系和条件下,从筛选出的20例直肠腺癌组织DNA中扩增出apc基因MCR的产物,而40例B-NHL组织DNA中未扩增出相应产物。结论apc抑癌基因第15外显子MCR突变是结、直肠腺癌等恶性肿瘤的常见分子改变,但在B-NHL中可能不是普遍现象并有可能发生纯合性缺失。Objective To analyze the effect of the mutation of the mutation cluster （MCR） region in the exon 15 of the apc on the pathogenesis of B-cell Non-Hodgkin lymphoma. Methods Mutation of MCR of apc gene was detected by PCR and agonose gel electrophoresis in tissues from 40 patients with B-cell non-Hodgkin lymphoma and 20 eases with rectal adenocareinoma （ used as positive controls）. Results Mutation of MCR of the apc gene was detected in all tissues from the 20 patients with rectal adenecarcinoma,while none of the tissues from the B-cell Non-Hodgkin lymphoma had this mutation. Conclusion Mutation of the MCR of the apc exon 15 is a common molecular alteration in rectal adenocareinoma. In B-cell Non-Hodgkin lymphoma,this mutation is rarely seen, but might be presented withhomozygous deletion.

关 键 词：非霍奇金淋巴瘤 apc抑癌基因 纯合性缺失 

分 类 号：R733.1[医药卫生—肿瘤]