N^＋注入中性蛋白酶高产菌株诱变选育的研究  

Mutation breeding of neutral protease-producing strain by N^+ implantation

作　　者：张敏[1] 赵丛[1] 路福平[1] 蔡兴旺[1] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室

出　　处：《浙江大学学报（农业与生命科学版）》2008年第3期245-248,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家高技术研究发展计划863"资助项目(2007AA02Z212)

摘　　要：利用低能N+注入技术对产中性蛋白酶的枯草芽孢杆菌(Bacillus subtilis)进行诱变选育,以提高该菌株的产酶能力.试验结果表明,菌株的存活率曲线是典型的马鞍型"剂量-效应曲线,在注入剂量为(30~50)×1014ions·cm-2的区域内具有高的正突变率.在注入能量为30keV、注入剂量为50×1014 ions·cm-2的条件下,经过筛选最终获得1株遗传稳定性良好的高产菌株NP131,其所产中性蛋白酶活力稳定在15230 U·mL-1左右,较出发菌株提高了71.2%.比较突变菌株NP131与出发菌株的产酶曲线,发现2条曲线的变化规律一致.说明离子注入技术是一种比较理想的微生物菌种选育方法.To increase the production of neutral protease, Bacillus subtilis was implanted with low energy N^＋ beam to breed mutants. The results indicate that the survival rate curve took a ＂saddle shape＂ and there was a high positive mutation rate by the dose of （30-50）× 10^14 ions· cm^-2. Under the implantation conditions of 30 keV and 50× 10^24 ions·cm^-2, strain NP131 was obtained with high yield and stability. The neutral protease yield produced by NP131 was about 15230 U·mL^-1, and increased by 71.2% than the original strain. The comparison of the neutral protease activity curve of NP131 and original strain showed that the regularity of the variation was similar. It was indicated that the ion implantation technique is a feasible method in breeding strains.

关 键 词：离子注入 中性蛋白酶 诱变选育 

分 类 号：Q933[生物学—微生物学]